Conditions for maintenance of hepatocyte differentiation and function in 3D cultures
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Primary Cell, Hepatocyte, Cell Culture
SUBMITTER: 
Niklas Handin
LAB HEAD: Per Artursson
PROVIDER: PXD024632 | Pride | 2022-02-17
REPOSITORIES: Pride
Publications
Conditions for maintenance of hepatocyte differentiation and function in 3D cultures.
Handin Niklas N Mickols Evgeniya E Ölander Magnus M Rudfeldt Jakob J Blom Kristin K Nyberg Frida F Senkowski Wojciech W Urdzik Jozef J Maturi Varun V Fryknäs Mårten M Artursson Per P
iScience 20211005 11
Spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. The cultures are maintained under different conditions, with possible confounding results. We performed an in-depth analysis of the influence of various culture conditions to find the optimal conditions for the maintenance of an <i>in vivo</i> like phenotype. The formation, protein expression, and function of PHH spheroids were followed for three weeks in a high-throughput 384-well f ...[more]
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