Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Caenorhabditis Elegans
TISSUE(S): Embryo
SUBMITTER: Berndt Muller
LAB HEAD: Berndt Muller
PROVIDER: PXD024763 | Pride | 2022-06-24
REPOSITORIES: Pride
Fasimoye Rotimi Yemi RY Spencer Rosie Elizabeth Barker REB Soto-Martin Eva E Eijlers Peter P Elmassoudi Haitem H Brivio Sarah S Mangana Carolina C Sabele Viktorija V Rechtorikova Radoslava R Wenzel Marius M Connolly Bernadette B Pettitt Jonathan J Müller Berndt B
Nucleic acids research 20220701 13
Spliced leader trans-splicing is essential for gene expression in many eukaryotes. To elucidate the molecular mechanism of this process, we characterise the molecules associated with the Caenorhabditis elegans major spliced leader snRNP (SL1 snRNP), which donates the spliced leader that replaces the 5' untranslated region of most pre-mRNAs. Using a GFP-tagged version of the SL1 snRNP protein SNA-1 created by CRISPR-mediated genome engineering, we immunoprecipitate and identify RNAs and protein c ...[more]