Proteomic analysis of exosomes isolated from S. Typhimurium-infected macrophages.

ABSTRACT: Exosomes derived from S. Typhimurium-infected RAW264.7 macrophages (24 and 48 hpi) were isolated by differential ultracentrifugation. Equal protein samples in triplicates were subjected to protein extraction and separation by SDS-PAGE. Protein bands excised from each lane were reduced, alkylated, and digested with trypsin. Peptide pools were desalted by C18 columns and analyzed by liquid chromatography-Orbitrap Fusion tandem mass spectrometry

INSTRUMENT(S):

ORGANISM(S): Salmonella Typhimurium (strain Lt2 / Sgsc1412 / Atcc 700720) Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Mariola Edelmann  

LAB HEAD: Mariola J Edelmann

PROVIDER: PXD024838 | Pride | 2021-04-02

REPOSITORIES: Pride