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Deep time-course proteomics of SARS-CoV and SARS-CoV-2 infected human lung epithelial cells (Calu-3) reveals strong induction of interferon-stimulated gene (ISG) expression by SARS-CoV-2 in contrast to SARS-CoV

ABSTRACT: In the present study, we used data-independent acquisition mass spectrometry (DIA-MS) to analyse the protein expression in Calu-3 cells infected with SARS-CoV and SARS-CoV-2 over the time-course of 24 hours. About 8400 proteins were identified, from which about 90% could be quantified across the experiment. This results in a deep and comprehensive proteome map, which reflects time-dependent protein expression changes during SARS-CoV and SARS-CoV-2 infections and provides deep insights into the virus-specific immunmodulation of human lung cells.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Severe Acute Respiratory Syndrome Coronavirus 2 Severe Acute Respiratory Syndrome-related Coronavirus

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Covid-19,Disease Free

SUBMITTER: Marica Grossegesse

LAB HEAD: Andreas Nitsche

PROVIDER: PXD024883 | Pride | 2022-01-20

REPOSITORIES: Pride

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