Dataset Information

Goat Peripheral Blood Mononuclear Cells (PBMCs) responses to infection vary with Peste des Petits Ruminants Virus virulence

ABSTRACT: Peste des Petits Ruminants (PPR) is a highly infectious disease caused by a virus of the genus Morbillivirus (PPRV), infecting mainly sheep and goat. Susceptibility of host can vary widely with host breed and virus strain. The mechanisms underlying this variability are not well understood. Here, we carried out the first comparative in vitro study on goat peripheral blood mononuclear cells (PBMCs) infected with PPRV strains of different virulence, vaccine strain (N75/1), low- (IC89), and high-virulent strain (MA08). Goat PBMCs were infected by the different strains and stimulated with Concanavalin A (Con A), and proteome changes of these cells were evaluated during the infection. The level of PPRV replication was assessed first by RT-qPCR quantification of viral N mRNA and by labelling the viral N protein inside the cells with specific antibodies and analysed by flow cytometry. The dynamics of PBMC sub-populations were also evaluated by flow cytometry. Our results showed that viral replication is critical for PPRV inhibitory effect on PBMCs proliferation. Highly virulent MA08 strain reached a higher level of replication in ConA-stimulated PBMCs and induced higher mortality compared to other strains. Low-virulent IC89 strain showed the lower replication level and cell proliferative inhibitory capacities. Differences in immune genes expression levels were assessed using RNAseq analysis and protein expression was compared using mass spectrometry. Analysis of these data provided the transcriptional and proteome landscape of PBMCs infected with these strains. The possible association between the transcriptional and proteome landscape and changes in immune cell subpopulations dynamics was explored.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Peste Des Petits Ruminants Virus Capra Hircus (goat)

TISSUE(S): Leukocyte

SUBMITTER: Functional Proteomics Platform FPP

LAB HEAD: Arnaud Bataille

PROVIDER: PXD024890 | Pride | 2021-11-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PPRPROT_200710_HF2_A2_1.raw	Raw
	PPRPROT_200710_HF2_A3_1.raw	Raw
	PPRPROT_200710_HF2_A4_1.raw	Raw
	PPRPROT_200710_HF_A2_3.raw	Raw
	PPRPROT_200710_HF_A2_4.raw	Raw

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Publications

Identification of Differential Responses of Goat PBMCs to PPRV Virulence Using a Multi-Omics Approach.

Eloiflin Roger-Junior RJ Auray Gaël G Python Sylvie S Rodrigues Valérie V Seveno Martial M Urbach Serge S El Koulali Khadija K Holzmuller Philippe P Totte Philippe P Libeau Genevieve G Bataille Arnaud A Summerfield Artur A

Frontiers in immunology 20211004

Peste des petits ruminants (PPR) is an acute transboundary infectious viral disease of small ruminants, mainly sheep and goats. Host susceptibility varies considerably depending on the PPR virus (PPRV) strain, the host species and breed. The effect of strains with different levels of virulence on the modulation of the immune system has not been thoroughly compared in an experimental setting so far. In this study, we used a multi-omics approach to investigate the host cellular factors involved in ...[more]

PMID: 34671358

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