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Pull-down assay to identify proteins interacting with the small protein AtpΘ (Norf1) in Synechocystis sp. PCC 6803

ABSTRACT: Small proteins in prokaryotes are usually defined as polypeptides that are shorter than 80 amino acids. In the model cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis 6803), the existence of several small proteins was verified previously. Among them, Norf1 (renamed to AtpΘ afterwards) is a small protein that is highly accumulated in darkness or stationary phase. To clarify the functions of AtpΘ, co-immunoprecipitation was performed to identify its interaction partners using FLAG-tagged AtpΘ protein as bait and FLAG-tagged sfGFP as negative control in three biological replicates each.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Synechocystis Sp. Pcc 6803

SUBMITTER: Sandra Maass

LAB HEAD: Dörte Becher

PROVIDER: PXD024905 | Pride | 2022-04-14

REPOSITORIES: Pride

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AtpΘ is an inhibitor of F<sub>0</sub>F<sub>1</sub> ATP synthase to arrest ATP hydrolysis during low-energy conditions in cyanobacteria.

Song Kuo K Baumgartner Desirée D Hagemann Martin M Muro-Pastor Alicia M AM Maaß Sandra S Becher Dörte D Hess Wolfgang R WR

Current biology : CB 20211110 1

Biological processes in all living cells are powered by ATP, a nearly universal molecule of energy transfer. ATP synthases produce ATP utilizing proton gradients that are usually generated by either respiration or photosynthesis. However, cyanobacteria are unique in combining photosynthetic and respiratory electron transport chains in the same membrane system, the thylakoids. How cyanobacteria prevent the futile reverse operation of ATP synthase under unfavorable conditions pumping protons while ...[more]

PMID: 34762820

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2016-06-16 | E-GEOD-83387 | biostudies-arrayexpress

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