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Cryptococcus neoformans proteins during fungal-macrophage interaction

ABSTRACT: Cryptococcus neoformans interactions with murine macrophages are critical for disease. In this project we analyzed fungal proteins which were co-purified with murine host proteins after interaction. H99 C. neoformans was opsonized with mAb 18B7 and addedd to murine macrophages. Then murine cells were lysed and cell extracts submitted to proteomics.

INSTRUMENT(S): orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Macrophage

SUBMITTER: Carolina Coelho

LAB HEAD: Arturo Casadevall

PROVIDER: PXD024951 | Pride | 2023-03-11

REPOSITORIES: Pride

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Cryptococcus neoformans releases proteins during intracellular residence that affect the outcome of the fungal-macrophage interaction.

Jung Eric H EH Park Yoon-Dong YD Dragotakes Quigly Q Ramirez Lia S LS Smith Daniel Q DQ Reis Flavia C G FCG Dziedzic Amanda A Rodrigues Marcio L ML Baker Rosanna P RP Williamson Peter R PR Jedlicka Anne A Casadevall Arturo A Coelho Carolina C

microLife 20220921

Cryptococcus neoformans is a facultative intracellular pathogen that can replicate and disseminate in mammalian macrophages. In this study, we analyzed fungal proteins identified in murine macrophage-like cells after infection with C. neoformans. To accomplish this, we developed a protocol to identify proteins released from cryptococcal cells inside macrophage-like cells; we identified 127 proteins of fungal origin in infected macrophage-like cells. Among the proteins identified wa ...[more]

PMID: 36247839

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