Human TRIM72 localizes on the microsome

ABSTRACT: Membrane repair machinery is a unique process to maintain cell integrity. In the early step of repair process, TRIM72 facilitates the patch membranes to damaged sites for resealing membrane and protects cells from acute membrane injury. The TRIM72 is an E3 ubiquitin ligase belonging to tripartite motif (TRIM) superfamily. Not only the repair machinery, the TRIM72 is also known as targeting membrane proteins such as insulin substrate receptor-1 (IRS-1) and insulin receptor (IR), so membrane binding events would be essential for TRIM72 to play a role. We found that the human TRIM72 localizes on the microsomal vesicles with very strong affinity. By the mass spectrometric gene ontology analysis, it has been confirmed the microsomal fractions were originated from the junctional and the exocytic vesicles. Moreover, identified proteins are classified to the cell adhesion and cell adhesion molecule binding. These results suggest that the cellular activity of TRIM72 would be related to vesicular proteins which engage to cell adhesion and furthermore, it should be mediated after membrane trans-localization of TRIM72.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sehwan Jang  

LAB HEAD: Hyun Kyu Song

PROVIDER: PXD024978 | Pride | 2024-01-26

REPOSITORIES: Pride