Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Permanent Cell Line Cell, Epithelial Cell, Cell Culture
DISEASE(S): Breast Cancer
SUBMITTER: Juan Oses-Prieto
LAB HEAD: Davide Ruggero
PROVIDER: PXD025018 | Pride | 2022-10-14
REPOSITORIES: Pride
Xu Yichen Y Huangyang Peiwei P Wang Ying Y Xue Lingru L Devericks Emily E Nguyen Hao G HG Yu Xiuyan X Oses-Prieto Juan A JA Burlingame Alma L AL Miglani Sohit S Goodarzi Hani H Ruggero Davide D
Cell 20210923 20
Estrogen receptor α (ERα) is a hormone receptor and key driver for over 70% of breast cancers that has been studied for decades as a transcription factor. Unexpectedly, we discover that ERα is a potent non-canonical RNA-binding protein. We show that ERα RNA binding function is uncoupled from its activity to bind DNA and critical for breast cancer progression. Employing genome-wide cross-linking immunoprecipitation (CLIP) sequencing and a functional CRISPRi screen, we find that ERα-associated mRN ...[more]