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Newly Synthesized protein after VapC5 induction in Mycobacterium abscessus ATCC19977

ABSTRACT: Analyzing newly synthesized protein after VapC5 induction in mycobacterium abscessus 19977

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mycobacteroides Abscessus Subsp. Abscessus

SUBMITTER: Unnati Chauhan

LAB HEAD: Nancy Woychik

PROVIDER: PXD025047 | Pride | 2023-07-20

REPOSITORIES: Pride

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Mycobacterium abscessus VapC5 toxin potentiates evasion of antibiotic killing by ribosome overproduction and activation of multiple resistance pathways.

Troian Eduardo A EA Maldonado Heather M HM Chauhan Unnati U Barth Valdir C VC Woychik Nancy A NA

Nature communications 20230622 1

Mycobacterium abscessus (Mab) infections are inexplicably intractable to clearing after aggressive and lengthy treatment regimens. Here we discovered that acquisition of a single toxin-antitoxin system enables Mab to activate a phenotypic switch that enhances survival upon treatment with current first-line antibiotics. This switch is tripped when the VapC5 toxin inactivates tRNA<sup>SerCGA</sup> by cleavage at only one site within its anticodon, leading to growth arrest. Concomitant tRNA<sup>Ser ...[more]

PMID: 37349306

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