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Proteomic analysis of primary tumor and lymph metastatic tissues as well as lung cancer cells

ABSTRACT: 4 pairs of matched primary versus metastatic tumor tissues from formalin-fixed, paraffin-embedded (FFPE) tissue bank,and two frozen lung tumor tissues from patients with primary orlymph metastasis. Then performed tandem mass tag (TMT) labeling and quantitative mass spectrometry analysis. A549 Cells we used was overexpressed luceiferase and designated as L0,underwent 3rounds selection in vivo to form L2, L6 that were metastatic to the spinal. We cultured L0 cells in "light" medium, L2,L6 in "medium heavy" and "heavy" respectively. Harvesting of cells and combined in 1:1:1 ratio, peptides were seperated using high-PH RPLC and analyzed by LC-MS/MS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Lymph Node

SUBMITTER: Lin Mao

LAB HEAD: Lujian Liao

PROVIDER: PXD025052 | Pride | 2022-02-17

REPOSITORIES: Pride

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Proteomic analysis of lung cancer cells reveals a critical role of BCAT1 in cancer cell metastasis.

Mao Lin L Chen Jin J Lu Xue X Yang Canlin C Ding Yi Y Wang Mingming M Zhang Yunpeng Y Tian Yuying Y Li Xing X Fu Yunyun Y Yang Yueying Y Gu Yunyun Y Gao Fei F Huang Junxing J Liao Lujian L

Theranostics 20210927 19

Metastasis is the major cause of high mortality in lung cancer. Exploring the underlying mechanisms of metastasis thus holds promise for identifying new therapeutic strategies that may enhance survival. <b>Methods:</b> We applied quantitative mass spectrometry to compare protein expression profiles between primary and metastatic lung cancer cells whilst investigating metastasis-related molecular features. <b>Results:</b> We discovered that BCAT1, the key enzyme in branched-chain amino acid metab ...[more]

PMID: 34646394

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