Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Embryonic Stem Cell
SUBMITTER: Enes Ugur
LAB HEAD: Heinrich Leonhardt
PROVIDER: PXD025053 | Pride | 2021-09-24
REPOSITORIES: Pride
Qin Weihua W Ugur Enes E Mulholland Christopher B CB Bultmann Sebastian S Solovei Irina I Modic Miha M Smets Martha M Wierer Michael M Forné Ignasi I Imhof Axel A Cardoso M Cristina MC Leonhardt Heinrich H
Nucleic acids research 20210701 13
Heterochromatin binding protein HP1β plays an important role in chromatin organization and cell differentiation, however the underlying mechanisms remain unclear. Here, we generated HP1β-/- embryonic stem cells and observed reduced heterochromatin clustering and impaired differentiation. We found that during stem cell differentiation, HP1β is phosphorylated at serine 89 by CK2, which creates a binding site for the pluripotency regulator KAP1. This phosphorylation dependent sequestration of KAP1 ...[more]