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Lrrc23 WT and KO whole sperm proteomic analysis

ABSTRACT: LRRC23 is required for stablizing the sperm axonemal structure. Mass spectrometry analysis was carried out to compare the proteome of Lrrc23 WT and KO sperm.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis, Sperm

SUBMITTER: Yonggang Lu

LAB HEAD: Masahito Ikawa

PROVIDER: PXD025166 | Pride | 2021-11-02

REPOSITORIES: Pride

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LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice.

Zhang Xin X Sun Jiang J Lu Yonggang Y Zhang Jintao J Shimada Keisuke K Noda Taichi T Zhao Shuqin S Koyano Takayuki T Matsuyama Makoto M Zhou Shushu S Wu Jiayan J Ikawa Masahito M Liu Mingxi M

Journal of cell science 20211021 20

Cilia and flagella are ancient structures that achieve controlled motor functions through the coordinated interaction based on microtubules and some attached projections. Radial spokes (RSs) facilitate the beating motion of these organelles by mediating signal transduction between dyneins and a central pair (CP) of singlet microtubules. RS complex isolation from Chlamydomonas axonemes enabled the detection of 23 radial spoke proteins (RSP1-RSP23), although the roles of some radial spoke proteins ...[more]

PMID: 34585727

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