Proteome of long-term expanded human CD4+ and CD8+ T cells

ABSTRACT: We analyzed the total proteome of CD4+ and CD8+ T cells isolated from human peripheral blood mononuclear cells (PBMC), and cultured to perform a CRISPR/CAS9 edition of their genome, in order to introduce an OST sequence at the C-terminus of proteins of interest (SLP76 or ZAP70, n=3 biological replicates in each case). Control T cells , isolated and cultured in the same way, but not modified by CRISPR/CAS9, were also analyzed (WT, n=3 or 6 biological replicates).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Blood

SUBMITTER: Anne Gonzalez de Peredo  

LAB HEAD: Odile Schiltz

PROVIDER: PXD025174 | Pride | 2022-01-10

REPOSITORIES: Pride