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Investigation on the quantitative proteomic responses of bloom-forming Cyanobacteria Microcystis aeruginosa to phosphorus depletion and starvation by the method of isobaric tags for relative and absolute quantitation (iTRAQ)

ABSTRACT: Microcystis aeruginosa cells were treated with phosphorus repletion, depletion and starvation. Isobaric tags for relative and absolute quantitation (iTRAQ) proteomic method was employed to explore to the effects of phosphorus limitation on Microcystis aeruginosa cells at the protein level. This investigation would contribute to the understanding of global cellular responses of Microcystis to phosphorus limitation and provide theoretical basis for deciding whether it is an effective way to control Microcystis blooms by phosphorus reduction.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Microcystis Aeruginosa

TISSUE(S): Photosynthetic Cell, Cell Culture

SUBMITTER: Nian Wei

LAB HEAD: Lirong Song

PROVIDER: PXD025188 | Pride | 2021-06-29

REPOSITORIES: Pride

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Quantitative Proteomic and Microcystin Production Response of Microcystis aeruginosa to Phosphorus Depletion.

Wei Nian N Song Lirong L Gan Nanqin N

Microorganisms 20210531 6

Microcystis blooms are the most widely distributed and frequently occurring cyanobacterial blooms in freshwater. Reducing phosphorus is suggested to be effective in mitigating cyanobacterial blooms, while the underlying molecular mechanisms are yet to be elucidated. In the present study, isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics was employed to study the effects of phosphorus depletion on Microcystis aeruginosa FACHB-905. The product ...[more]

PMID: 34072711

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