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SARS-CoV-2 detection from clinical samples

ABSTRACT: Shotgun analysis of SARS-CoV-2 clinical samples previously characterized by RT-PCR.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human) Severe Acute Respiratory Syndrome Coronavirus 2

TISSUE(S): Nasopharynx Epithelium

SUBMITTER: VALDEMIR CARVALHO

LAB HEAD: Valdemir Melechco Carvalho

PROVIDER: PXD025214 | Pride | 2021-04-07

REPOSITORIES: Pride

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Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts.

Cardozo Karina Helena Morais KHM Lebkuchen Adriana A Okai Guilherme Gonçalves GG Schuch Rodrigo Andrade RA Viana Luciana Godoy LG Olive Aline Nogueira AN Lazari Carolina Dos Santos CDS Fraga Ana Maria AM Granato Celso Francisco Hernandes CFH Pintão Maria Carolina Tostes MCT Carvalho Valdemir Melechco VM

Nature communications 20201203 1

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is pressing public health systems around the world, and large population testing is a key step to control this pandemic disease. Here, we develop a high-throughput targeted proteomics assay to detect SARS-CoV-2 nucleoprotein peptides directly from nasopharyngeal and oropharyngeal swabs. A modified magnetic particle-based proteomics approach implemented on a robotic liquid handler enables fully automated preparation of 9 ...[more]

PMID: 33273458

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