Ontology highlight
ABSTRACT:
INSTRUMENT(S): Synapt MS
ORGANISM(S): Escherichia Coli
SUBMITTER: Brenda Rosa
LAB HEAD: Kasper Dyrberg Rand
PROVIDER: PXD025300 | Pride | 2021-06-07
REPOSITORIES: Pride
Molecular & cellular proteomics : MCP 20210519
CysE and CysK, the last two enzymes of the cysteine biosynthetic pathway, engage in a bienzyme complex, cysteine synthase, with yet incompletely characterized three-dimensional structure and regulatory function. Being absent in mammals, the two enzymes and their complex are attractive targets for antibacterial drugs. We have used hydrogen/deuterium exchange MS to unveil how complex formation affects the conformational dynamics of CysK and CysE. Our results support a model where CysE is present i ...[more]