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Proteomic profile of Pinus pinaster roots under ammonium nutrition

ABSTRACT: Ammonium nutrition was studied at short times (24 hours) in roots from one month-old seedlings of maritime pine. Control seedlings were irrigated with 80 mL of water (C) and the experimental seedlings with 80 mL of 3 mM NH4Cl. Root samples were collected at 24 hours post-irrigation and immediately frozen in liquid N. This experiment was carried out three independent times.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Pinus Pinaster (maritime Pine)

TISSUE(S): Plant Cell, Root

SUBMITTER: César Lobato

LAB HEAD: Rafael A. Cañas

PROVIDER: PXD025331 | Pride | 2023-03-11

REPOSITORIES: Pride

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Epitranscriptome changes triggered by ammonium nutrition regulate the proteome response of maritime pine roots.

Ortigosa Francisco F Lobato-Fernández César C Pérez-Claros Juan Antonio JA Cantón Francisco R FR Ávila Concepción C Cánovas Francisco M FM Cañas Rafael A RA

Frontiers in plant science 20221222

Epitranscriptome constitutes a gene expression checkpoint in all living organisms. Nitrogen is an essential element for plant growth and development that influences gene expression at different levels such as epigenome, transcriptome, proteome, and metabolome. Therefore, our hypothesis is that changes in the epitranscriptome may regulate nitrogen metabolism. In this study, epitranscriptomic modifications caused by ammonium nutrition were monitored in maritime pine roots using Oxford Nanopore Tec ...[more]

PMID: 36618661

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