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Comparative proteomic profiling reveals specific adaptation of Vibrio anguillarum to oxidative stress, iron deprivation and humoral components of innate immunity

ABSTRACT: The gram-negative bacterium Vibrio (Listonella) anguillarum is the causative agent of vibriosis, a bacterial disease affecting aquacultural industry across the globe. The full mechanism of V. anguillarum pathogenesis is not completely understood, but many virulence determinants have been identified. The current study aimed to obtain molecular insights into the proteome response of the bacterium to several conditions mimicking vibriosis. Our data shed light on the adaptability of V. anguillarum to oxidative stress, iron limitations and the complement system, and offer potential virulence determinants associated in particular with septicemia over the course of vibriosis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Vibrio Anguillarum

SUBMITTER: Magnus Arntzen

LAB HEAD: Gustav Vaaje-Kolstad

PROVIDER: PXD025367 | Pride | 2021-11-25

REPOSITORIES: Pride

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Project description:The highly infectious bacterium Francisella tularensis is a facultative intracellular pathogen, whose virulence requires proliferation inside host cells, including macrophages. Although some Francisella determinants of intracellular growth have been identified, much remains to be understood about the pathogenesis of this organism. In particular, how Francisella responds to its intracellular environment could provide clues about its intracellular biology and reveal pathogenic determinants based on their intracellular expression profiles. Here we have performed a global transcriptional profiling of the highly virulent F. tularensis subsp. tularensis Schu S4 strain during its intracellular cycle within primary murine macrophages. Phagocytosed bacteria rapidly responded to their intracellular environment and progressively altered their transcriptional profile over time. Differential gene expression profiles were revealed that correlated with specific intracellular locations of the bacteria. Upregulation of general and oxidative stress response genes was a hallmark of the early phagosomal and late endosomal stages, while induction of a subset of transport and metabolic genes characterized the cytosolic replication stages. Expression of the Francisella Pathogenicity Island (FPI) genes, the functions of which are associated with intracellular proliferation, increased during the intracellular cycle. Similarly, 27 chromosomal loci putatively encoding hypothetical, secreted, outer membrane proteins or transcriptional regulators were identified as upregulated during the intracellular cycle. In-frame deletion of FTT0383, the Schu S4 ortholog of fevR, of FTT0369c or FTT1676 abolished the ability of Schu S4 to either survive or proliferate intracellularly, demonstrating that bacterial factors of intracellular pathogenesis can be identified based on their intracellular expression profile. In conclusion, establishing the intracellular transcriptome of Francisella has revealed important aspects of its intracellular biology and identified novel virulence determinants of this pathogen. Keywords: Time series Intracellular cycle within primary murine macrophages using the time series of zero, one, two, four, eight, twelve, sixteen and twenty-four hours

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Comparative proteomic profiling reveals specific adaptation of Vibrio anguillarum to oxidative stress, iron deprivation and humoral components of innate immunity

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