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Identification of CARP3-interacting proteins in Trypanosoma brucei bloodstream forms and procyclic forms

ABSTRACT: How flagellar signaling regulates the host interaction of parasites remains a challenge due to poor conservation of signaling systems with those in cilia of higher organisms. The trypanosome-specific cAMP response protein 3 (CARP3) shows developmentally regulated localization at the flagellar tip membrane, where it is essential for parasite swarming and colonization of the tsetse fly insect vector. This project describes identification of CARP3-YFP interacting proteins by GFP trap pull down followed by mass spectrometry in the bloodstream stage as well as in the procyclic stage of Trypanosoma brucei.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Trypanosoma Brucei Brucei

SUBMITTER: Ignasi Forne

LAB HEAD: Boshart, Michael

PROVIDER: PXD025398 | Pride | 2022-08-31

REPOSITORIES: Pride

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A multi-adenylate cyclase regulator at the flagellar tip controls African trypanosome transmission.

Bachmaier Sabine S Giacomelli Giacomo G Calvo-Alvarez Estefanía E Vieira Larissa Rezende LR Van Den Abbeele Jan J Aristodemou Aris A Lorentzen Esben E Gould Matt K MK Brennand Ana A Dupuy Jean-William JW Forné Ignasi I Imhof Axel A Bramkamp Marc M Salmon Didier D Rotureau Brice B Boshart Michael M

Nature communications 20220916 1

Signaling from ciliary microdomains controls developmental processes in metazoans. Trypanosome transmission requires development and migration in the tsetse vector alimentary tract. Flagellar cAMP signaling has been linked to parasite social motility (SoMo) in vitro, yet uncovering control of directed migration in fly organs is challenging. Here we show that the composition of an adenylate cyclase (AC) complex in the flagellar tip microdomain is essential for tsetse salivary gland (SG) colonizat ...[more]

PMID: 36114198

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Identification of ESAG4-interacting proteins in Trypanosoma brucei bloodstream forms

Project description:How flagellar signaling regulates the host interaction of parasites remains a challenge due to poor conservation of signaling systems with those in cilia of higher organisms. Flagellar cAMP signaling is implicated in interaction of the unicellular parasite Trypanosoma brucei with its hosts. The second messenger is produced by a large and atypical family of receptor-type adenylate cyclases (ACs) with unknown regulation mechanism. In order to identify trypanosome AC interacting proteins, ESAG4, an AC highly abundant in the parasite’s bloodstream stage, was fused to GFP and purified via GFP trap pull down followed by mass spectrometry.

2022-08-31 | PXD025412 | Pride

Quantitative proteomics upon CARP3 knock down in Trypanosoma brucei bloodstream forms

Project description:How flagellar signaling regulates the host interaction of parasites remains a challenge due to poor conservation of signaling systems with those in cilia of higher organisms. The trypanosome-specific cAMP response protein 3 (CARP3) shows developmentally regulated localization at the flagellar tip membrane, where it is essential for parasite swarming and colonization of the tsetse fly insect vector. This project describes a label-free, quantitative proteomics approach that identifies proteins changing in abundance upon inducible CARP3 knock down in bloodstream stage Trypanosoma brucei.

2022-08-31 | PXD025401 | Pride

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Project description:RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.

2015-09-11 | PXD002880 | Pride

H2AZ extended acidic patch is necessary for formation specialized chromatin states in ESCs [RNA-Seq]

Project description:The H2A variant H2AZ is essential for embryonic development and for proper execution of developmental gene expression programs in embryonic stem cells (ESCs). Divergent regions in H2AZ are likely key for its functional specialization, but we know little about how these differences contribute to chromatin regulation. Here, we show that the extended acidic patch, specifically the three divergent residues in the C-terminal docking domain, is necessary for lineage commitment during ESC differentiation and proper execution of gene expression programs during ESC differentiation. Surprisingly, disruption of the acidic patch domain has a distinct consequence on cellular specification compared to H2AZ depletion. This is consistent with differences in gene expression profiles of H2AZ M-bM-^@M-^Sdepleted and acidic patch (AP) mutant ESCs during early lineage commitment. Interestingly, the distinct consequence of AP mutant expression on gene regulation is coincidence with an altered destabilized chromatin state and high chromatin mobility dependent on active transcription. Collectively, our data shows that the divergent residues within the acidic patch domain are key structural determinants of H2AZ function and links chromatin structure and dynamics with gene regulation and cell fate specification. H2AZ extended acidic patch was mutated, or H2AZ was KD in mouse embryonic stem cells and RNA-Seq analysis was performed on the resulting cultures. Characterization of H2AZ-WT and -AP3-mutant binding specificities were performed by ChIP-Seq.

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Release of the plant peptide hormone PEP1 from its precursor PROPEP1 in response to physical damage is due to Ca2+-dependent activation of METACASPASE4.

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2019-04-18 | PXD010816 | Pride

Novel sterol metabolic network of Trypanosoma brucei procyclic and bloodstream forms.

Project description: Not available

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Project description: Not available

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2023-06-27 | PXD038414 | Pride

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2023-01-30 | PXD034335 | Pride

The histone acetyltransferase KAT6A is recruited to unmethylated CpG islands via a DNA-binding winged helix domainThe histone acetyltransferase KAT6A is recruited to unmethylated CpG islands via a DNA-binding winged helix domain

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