Dataset Information

Proteomic analysis of membrane vesicles from clinical Cutibacterium acnes isolates

ABSTRACT: Recently the membrane vesicles (MVs) production has been observed in Gram-positive bacterium, Cutibacterium acnes (C. acnes). In order to explore the mechanism of antibiotic resistance and the virulent components within the C. acnes-derived MVs, we isolated MVs from the clinical C. acnes, which were sensitive or resistant to antibiotics erythromycin and clindamycin. With the LC-MS/MS method, we detected several lipases, virulent factors and cell division protein differentially expressed between the sensitive and the resistant C. acnes-derived MVs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Cutibacterium Acnes Sk137

TISSUE(S): Cell Culture

SUBMITTER: Min Jiang

LAB HEAD: Ying Ma

PROVIDER: PXD025460 | Pride | 2022-05-20

REPOSITORIES: Pride

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Publications

Comparative Proteomic Analysis of Membrane Vesicles from Clinical <i>C. acnes</i> Isolates with Differential Antibiotic Resistance.

Jiang Min M Fan Xiaoyao X Jiang Ziqi Z Chen Huyan H Liu Ye Y Yu Tianze T Huang Qiong Q Ma Ying Y

Clinical, cosmetic and investigational dermatology 20220417

<h4>Purpose</h4><i>Cutibacterium acnes</i> (<i>C. acnes</i>) is closely associated with the pathogenesis of acne, and antibiotics targeting <i>C. acnes</i> have been widely used for decades. However, antibiotic resistance has been increasing rapidly. Membrane vesicles (MVs) have been found to play important roles in antibiotic resistance in some bacteria. We aimed to explore the mechanism of antibiotic resistance and the virulence components within <i>C. acnes</i>-derived MVs.<h4>Materials and m ...[more]

PMID: 35463830

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Project description:Periodontitis affects 47.1% of adult population in the U.S. Porphyromonas gingivalis is an opportunistic oral pathogen that colonizes the oral mucosa, invades myeloid dendritic cells and accesses the bloodstream, brain, placenta and other organs in human with periodontitis. Periodontitis also sustains a chronic long-term pro-inflammatory immune disorder, potentially contributing to other systemic conditions such as cardiovascular disease, type 2 diabetes mellitus, adverse pregnancy outcomes, and osteoporosis. However, the role of P. gingivalis minor and major fimbriae in DC-SIGN-TLR2 crosstalk during traverses from oral mucosa to these distant sites and its influence on survival of P. gingivalis within DCs and its immune-mechanism involve at molecular/transcriptome level has not been examined. In this study to address the role of fimbriae we utilized defined bacterial mutants that solely express minor fimbriae (Mfa1+Pg), major fimbriae (FimA+Pg) or are deficient in both fimbriae (MFB) and compared with un-infected control. P. gingivalis strains were maintained anaerobically (10% H2, 10% CO2, and 80% N2) in a Forma Scientific anaerobic system glove box model 1025/1029 at 37Â°C in Difco anaerobe broth MIC. Mutant strains were maintained using erythromycin (5 Î¼g/ml) for mutant Mfa1+Pg, tetracycline (2 Î¼g/ml) for mutant FimA+Pg and both erythromycin and tetracycline for double fimbriae mutant MFB. Bacterial suspensions were washed five times in PBS and re-suspended for spectrophotometer reading at OD 660 nm of 0.11, which previously determined to be equal to 5 x 107 CFU. For bacterial CFSE staining, the suspension were washed (3 times) and re-suspended in 5 Î¼M of CFSE in PBS. The bacteria were incubated for 30 min at 37Â°C in the dark. MoDCs were pulsed with Pg381, Mfa1+Pg, FimA+Pg and MFB at 10 MOI and incubated with the MoDCs for 12 hours and each experimental condition was performed in triplicate. To facilitate our understanding on host immunity and defense mechanism of this pathogen, here we used the Illumina High-throughput RNA-seq transcriptome profiling to investigate the myeloid dendritic cells response to oral Amphibiont (1. Pg381, 2. Mfa1+Pg, 3. FimA+Pg, 4. MFB and 5. Un-infected control group).

Dataset Information

Proteomic analysis of membrane vesicles from clinical Cutibacterium acnes isolates

Publications

Comparative Proteomic Analysis of Membrane Vesicles from Clinical <i>C. acnes</i> Isolates with Differential Antibiotic Resistance.

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