Proteomic Analysis of rattus norvegicus colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea by label-free LC–MS/MS

ABSTRACT: To investigate the molecular pathological mechanisms of irritable bowel syndrome with diarrhea (IBS-D) and elucidate the effects of acupuncture on IBS-D colonic mucosa protein abundance in rats, a label-free high-throughput liquid chromatography-tandem mass spectrometry (LC-MS)-based proteomics analysis was used to survey the global changes of colonic mucosa proteins between different groups. A Nano flow Ultimate 3000 HPLC (Dionex Corp, Sunnyvale, CA) coupled online to a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) was used in this project.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Colonic Mucosa

DISEASE(S): Irritable Bowel Syndrome

SUBMITTER: Jing Liu  

LAB HEAD: Jia Li

PROVIDER: PXD025558 | Pride | 2022-10-14

REPOSITORIES: Pride