Proteomics informed by transcriptomics for a qualitative and quantitative analysis of the sialoproteome of Ornithodoros moubata adult ticks. SWATH Spectral Library building

ABSTRACT: The specific aim of the present work is to expand the data on the salivary proteome of O. moubata adult ticks, particularly of female ticks, by solving the aforementioned drawbacks, as part of the in depth characterization and analysis of the O. moubata sialome. Knowing this sialome will allow identifying and selecting novel salivary antigens as targets for tick vaccines, and their subsequent testing in animal immunization trials. We pay special attention to female ticks because they are a key developmental stage in the tick life cycle and vaccines may exert on them double deleterious effects, for instance an increase of mortality and a reduction/inhibition of the reproductive performance. With that aim, in the current work we have analysed samples of female and male saliva separately using two different mass spectrometry approaches: data-dependent acquisition (DDA) LC-MS/MS and Sequential Window Acquisition of all Theoretical fragment ion spectra Mass Spectrometry (SWATH MS). This last technique is a specific variant of data-independent acquisition (DIA) methods and is emerging as a technology that combines deep proteome coverage capabilities with quantitative consistency and accuracy.

INSTRUMENT(S):

ORGANISM(S): Ornithodoros Moubata

TISSUE(S): Saliva

SUBMITTER: Luz Valero  

LAB HEAD: Ana Oleaga Pérez

PROVIDER: PXD025660 | Pride | 2021-11-18

REPOSITORIES: Pride