Proteomics

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Protein composition of cobweb spiders gumfoot glue droplets

ABSTRACT: Orb weavers place aggregate glue on a stretchy capture spiral, whereas cobweb weavers add it to the ends of strong, stiff fibers, called gumfoot lines. We describe the quantitative proteomics of the aggregate glues of two cobweb weaving species, the Western black widow, Latrodectus hesperus, and the common house spider, Parasteatoda tepidariorum. For each species respectively, we identified 48 and 33 proteins that were significantly more abundant in the portion of the gumfoot line with glue than in its fibers. These proteins were highly enriched for glycosylation and phosphorylation relative to proteins found in silk fibers. Most enriched proteins were of anterior aggregate gland origin, supporting the hypothesis that cobweb weavers’ posterior aggregate glue is specialized for another function..

INSTRUMENT(S): Q Exactive

ORGANISM(S): Parasteatoda Tepidariorum Latrodectus Hesperus (western Black Widow Spider)

SUBMITTER: Thomas Clarke  

LAB HEAD: Nadia Ayoub

PROVIDER: PXD025685 | Pride | 2022-02-17

REPOSITORIES: Pride

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Publications

Protein Composition and Associated Material Properties of Cobweb Spiders' Gumfoot Glue Droplets.

Ayoub Nadia A NA   Friend Kyle K   Clarke Thomas T   Baker Richard R   Correa-Garhwal Sandra M SM   Crean Andrew A   Dendev Enkhbileg E   Foster Delaney D   Hoff Lorden L   Kelly Sean D SD   Patterson Wade W   Hayashi Cheryl Y CY   Opell Brent D BD  

Integrative and comparative biology 20211001 4

The origin of aggregate silk glands and their production of wet adhesive silks is considered a key innovation of the Araneoidea, a superfamily of spiders that build orb-webs and cobwebs. Orb-web weavers place aggregate glue on an extensible capture spiral, whereas cobweb weavers add it to the ends of strong, stiff fibers, called gumfoot lines. Here we describe the material behavior and quantitative proteomics of the aggregate glues of two cobweb weaving species, the western black widow, Latrodec  ...[more]

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