Name: Analysis of interactions with truncated cytoplasmic dynein-2 intermediate chain WDR34-Q158*
Keywords: Human

OmicsDI

Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of interactions with truncated cytoplasmic dynein-2 intermediate chain WDR34-Q158*

ABSTRACT: Analysis of proteins interactiong with the trucated form of the cytoplasmic dynein-2 intermediate chain WDR34-Q158* using GFP-trap

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Asphyxiating Thoracic Dystrophy

SUBMITTER: David Stephens

LAB HEAD: David Stephens

PROVIDER: PXD025686 | Pride | 2022-09-06

REPOSITORIES: pride

ACCESS DATA

Json Xml

Similar Datasets

Disease-associated mutations in WDR34 lead to diverse impacts on the assembly and function of dynein-2

Project description:The primary cilium is a sensory organelle, receiving signals from the external environment and relaying them into the cell. Mutations in proteins required for transport in the primary cilium lead to ciliopathies, a group of genetic disorders that commonly lead to the malformation of organs such as the kidney, liver and eyes and skeletal deformation. Motor proteins dynein-2 and kinesin-2 mediate retrograde and anterograde transport in the cilium. WDR34, a dynein-2 intermediate chain, is required for the maintenance of cilia function. Here, we investigated WDR34 mutations, some as compound mutations with other dynein-2 or IFT proteins, identified in Jeune syndrome, Short-Rib Polydactyly Syndrome or Asphyxiating thoracic dysplasia patients by stably expressing the mutant proteins in WDR34 knockout cells. WDR34 mutations led to different spectrums of phenotypes. Here, we have used quantitative proteomics demonstrated changes in dynein-2 assembly. These data re[resent pilot experiments subsequently developed fully and deposited as PXD032758.

2024-04-15 | PXD046827 | Pride

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET1

Project description:To identify those proteins interacting with the cytoplasmic dynein-2 using mammalian cell lines stably expressing HA-tagged intermediate chain subunits, WDR34 (DYNC2I2) or WDR60 (DYNC2I1).

2023-01-24 | PXD031151 | Pride

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET2

2023-01-24 | PXD031153 | Pride

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET1

2023-01-24 | PXD031154 | Pride

Analysis of impact of clinical mutations in WDR34 by GFP-trap proteomics.

2022-09-06 | PXD032758 | Pride

Protein interactions with GFP-tagged cytoplasmic dynein-2 DATASET2

Project description:To identify those proteins interacting with the cytoplasmic dynein-2 using mammalian cell lines stably expressing GFP-tagged intermediate chain subunits, WDR34 (DYNC2I2) or WDR60 (DYNC2I1).

2023-01-24 | PXD031158 | Pride

Protein interactions with GFP-tagged cytoplasmic dynein-2 DATASET1

2023-01-24 | PXD031157 | Pride

Protein interactions with GFP-tagged cytoplasmic dynein-2 DATASET3

2023-01-24 | PXD031156 | Pride

Protein interactions with HA-tagged WDR60 in CEP170 knockout cells

Project description:To identify those proteins interacting with the WDR60 subunit of cytoplasmic dynein-2 using mammalian cell lines stably expressing HA-tagged intermediate chain subunit WDR60 (DYNC2I1).

2024-04-15 | PXD046844 | Pride

Human hTERT-RPE1 cell AP-MS Dynein-2 intermediate chains

Project description:Affinity isolation of proteins interacting with dynein-2 intermediate chains WDR34 and WDR6 in both WT and WDR34/WDR60 knockout cell lines0

2018-11-04 | PXD010398 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data