The MICOS-MIB complex member PERM1 connects mitochondria with the sarcolemma via ankyrin B

ABSTRACT: Skeletal muscle subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria subpopulations have distinct metabolic activity and sensitivity, though the mechanisms that localize SSM to peripheral areas of muscle fibers are poorly understood. A protein interaction study and complexome profiling identified PERM1 interacts with the MICOS-MIB complex. Ablation of Perm1 in mice reduced muscle force, decreased mitochondrial membrane potential and complex I activity, and reduced the numbers of SSM in skeletal muscle. We demonstrate PERM1 interacts with the intracellular adaptor protein ankyrin B (ANKB) that connects the cytoskeleton to the plasma membrane. In addition, we discovered a C-terminal transmembrane helix that anchors PERM1 into the outer mitochondrial membrane. We conclude PERM1 functions in the MICOS-MIB complex and acts as an adapter to connect the mitochondria with the sarcolemma via ANKB.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Heart, Brain, Cell Culture, Muscle

SUBMITTER: Theresa Bock  

LAB HEAD: Marcus Krüger

PROVIDER: PXD025745 | Pride | 2021-11-16

REPOSITORIES: Pride