Dataset Information

Effect of soybean agglutinin on biological function of gut

ABSTRACT: This study aims to investigate the effects of SBA on the cell cycle, apoptosis, and to verify the mechanism of SBA anti-nutritional characters based on proteomic-based analysis. The IPEC-J2 cell line was cultured with medium containing 0.0, 0.5 or 2.0 mg/mL SBA. With increasing SBA levels, the percentage of the cells at G0/G1 phase, cell apoptosis rates, expressions of Bax and p21, and the activities of Casp-3 and Casp-9 were increased, while cyclin D1 and Bcl-2 expressions were declined (p<0.05). The proteomic analysis showed that the numbers of differentially expressed proteins, induced by SBA, were mainly enriched in different pathways including DNA replication, base excision repair, nucleus excision repair, mismatch repair, amide and peptide biosynthesis, ubiquitin-mediated proteolysis, as well as structures and functions of mitochondria and ribosome. In conclusion, the anti-nutritional mechanism of SBA is a complex cellular process. Such process including DNA related activities; protein synthesis and metabolism; signal-conducting relation; as well as subcellular structure and function. This study provides comprehensive information to understand the toxic mechanism of SBA in monogastrics.

INSTRUMENT(S):

ORGANISM(S): Enterococcus Faecalis (streptococcus Faecalis) Acyrthosiphon Pisum (pea Aphid) Heliocidaris Erythrogramma (sea Urchin) Yarrowia Lipolytica (candida Lipolytica) Pan Troglodytes (chimpanzee) Pinus Radiata (monterey Pine) Helicobacter Pylori J99 (campylobacter Pylori J99) Halobacterium Salinarum Merluccius Bilinearis (silver Hake) Drosophila Melanogaster (fruit Fly) Hepatitis C Virus Subtype 1a Pongo Pygmaeus (bornean Orangutan) Ictalurus Punctatus (channel Catfish) Hordeum Vulgare (barley) Trypanosoma Brucei Arabidopsis Thaliana (mouse-ear Cress) Gadus Morhua (atlantic Cod) Rattus Norvegicus (rat) Caenorhabditis Elegans Solanum Tuberosum (potato) Arachis Hypogaea (peanut) Vigna Mungo (rice Bean) (black Gram) Nicotiana Tabacum (common Tobacco) Homo Sapiens (human) Bos Taurus (bovine) Gallus Gallus (chicken) Cicer Arietinum (chickpea) (garbanzo) Mus Musculus (mouse) Bombyx Mori (silk Moth) Candida Albicans (yeast) Brassica Oleracea Var. Botrytis (cauliflower) Taeniopygia Guttata (zebra Finch) (poephila Guttata) Saccharomyces Cerevisiae (baker's Yeast) Xenopus Laevis (african Clawed Frog) Sus Scrofa Domesticus (domestic Pig) Escherichia Coli Struthio Camelus (ostrich) Brassica Napus (rape) Staphylococcus Aureus Ricinus Communis Zea Mays (maize) Dictyostelium Discoideum (slime Mold) Olea Europaea (common Olive) Equus Caballus (horse) Pisum Sativum (garden Pea) Danio Rerio (zebrafish) (brachydanio Rerio) Oryza Sativa (rice) Rattus Rattus (black Rat) Triticum Aestivum (wheat) Macaca Mulatta (rhesus Macaque) Mesocricetus Auratus (golden Hamster) Cercopithecus Aethiops (green Monkey) (grivet) Vitis Vinifera (grape) Apis Mellifera (honeybee)