Proteomics

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MDA-MB-231 MS analysis of ISGylome, GDI2 interactome and total proteome changes in different ISGylation models

ABSTRACT: Analysis of ISG15 conjugation (Isgylation)role as driver of poor prognosis in non HER2+ breast cancer tumours. The project includes an analysis of total proteome and ISGylome characterization of MDA-MB-231 cells, wild-type or knock-out for the ISGyltaion associated genes, ISG15, UBE2L6 and USP18. Additionally interactome analysis of the ISGylation target GDI2 was perfored in the same lines.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Alfonso Bolado  

LAB HEAD: Alexander von Kriegsheim

PROVIDER: PXD025753 | Pride | 2021-11-03

REPOSITORIES: Pride

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Publications

ISG15 is an ubiquitin-like modifier that is associated with reduced survival rates in breast cancer patients. The mechanism by which ISG15 achieves this however remains elusive. We demonstrate that modification of Rab GDP-Dissociation Inhibitor Beta (GDI2) by ISG15 (ISGylation) alters endocytic recycling of the EGF receptor (EGFR) in non-interferon stimulated cells using CRISPR-knock out models for ISGylation. By regulating EGFR trafficking, ISGylation enhances EGFR recycling and sustains Akt-si  ...[more]

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