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A novel method for creating a synthetic L-DOPA proteome and in vitro evidence of incorporation.

ABSTRACT: Proteinopathies are protein misfolding diseases that have an underlying factor that effect the conformation of proteoforms. A factor hypothesised to play a role in these diseases is the incorporation of non-protein amino acids into proteins, with a key example being the therapeutic drug levodopa. The presence of levodopa as a protein constituent has been explored in several studies but it has not been examined in a global proteomic manner. This paper provides a proof-of-concept method for enzymatically creating levodopa containing proteins using the enzyme tyrosinase, provides spectral evidence of in vitro incorporation and the induction of the unfolded protein response due to levodopa.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Cell Culture

SUBMITTER: Joel Steele

LAB HEAD: Kenneth J Rodgers

PROVIDER: PXD025759 | Pride | 2022-02-17

REPOSITORIES: Pride

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A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation.

Steele Joel Ricky JR Strange Natalie N Rodgers Kenneth J KJ Padula Matthew P MP

Proteomes 20210524 2

Proteinopathies are protein misfolding diseases that have an underlying factor that affects the conformation of proteoforms. A factor hypothesised to play a role in these diseases is the incorporation of non-protein amino acids into proteins, with a key example being the therapeutic drug levodopa. The presence of levodopa as a protein constituent has been explored in several studies, but it has not been examined in a global proteomic manner. This paper provides a proof-of-concept method for enzy ...[more]

PMID: 34073856

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Dataset Information

A novel method for creating a synthetic L-DOPA proteome and in vitro evidence of incorporation.

Publications

A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation.

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