A novel method for creating a synthetic L-DOPA proteome and in vitro evidence of incorporation.

ABSTRACT: Proteinopathies are protein misfolding diseases that have an underlying factor that effect the conformation of proteoforms. A factor hypothesised to play a role in these diseases is the incorporation of non-protein amino acids into proteins, with a key example being the therapeutic drug levodopa. The presence of levodopa as a protein constituent has been explored in several studies but it has not been examined in a global proteomic manner. This paper provides a proof-of-concept method for enzymatically creating levodopa containing proteins using the enzyme tyrosinase, provides spectral evidence of in vitro incorporation and the induction of the unfolded protein response due to levodopa.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Cell Culture

SUBMITTER: Joel Steele  

LAB HEAD: Kenneth J Rodgers

PROVIDER: PXD025759 | Pride | 2022-02-17

REPOSITORIES: Pride