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Differential protein expression in berry skin from red grapes with varying hybrid character

ABSTRACT: Protein expression from berry skin of four different red grape biotypes was compared at a proteome-wide level by bottom-up shotgun proteomics, label free quantification and MaxQuant-assisted computational analysis. Red grapes were from a purebred Vitis vinifera (Aglianico cv.), a V. vinifera (local Sciascinoso cv.) grafted onto an American rootstock, an interspecific hybrid (V. vinifera × V. labrusca, Isabel) and an uncharacterized red grape with some hybrid lineage, as demonstrated by the presence of relatively high amounts of anthocyanidin 3,5-O-diglucosides. The aim was assessing the differences among red grape biotypes at a protein expression levels, also addressing the possible effect of the grafting on the phenotypic expression of some key metabolic enzymes in grape berries.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Vitis Vinifera (grape)

TISSUE(S): Skin

SUBMITTER: Gianluca Picariello

LAB HEAD: Gianluca Picariello

PROVIDER: PXD025784 | Pride | 2022-02-22

REPOSITORIES: Pride

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Differential Protein Expression in Berry Skin from Red Grapes with Varying Hybrid Character.

Spada Valentina V Di Stasio Luigia L Ferranti Pasquale P Addeo Francesco F Mamone Gianfranco G Picariello Gianluca G

International journal of molecular sciences 20220119 3

Protein expression from the berry skin of four red grape biotypes with varying hybrid character was compared at a proteome-wide level to identify the metabolic pathways underlying divergent patterns of secondary metabolites. A bottom-up shotgun proteomics approach with label-free quantification and MaxQuant-assisted computational analysis was applied. Red grapes were from (i) purebred Vitis vinifera (Aglianico cv.); (ii) V. vinifera (local Sciascinoso cv.) grafted ont ...[more]

PMID: 35162980

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