An in situ ascorbate peroxidase 2-based labeling method and its application in detecting exosome proteins released from mouse proximal tubule cells under oxidative stress

ABSTRACT: Here, we developed an in situ labeling approach to identify exosome-loaded proteins, which bypasses the traditional exosome isolation steps. In this method, an engineered ascorbate peroxidase targeting to endosomes-destined exosomes was used to label proteins loaded inside exosomes released from kidney proximal tubule-derived cells. Mass spectrometry analysis of the kidney proximal tubule-derived cells identified oxidative stress-induced exosome proteome alteration.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Kidney

DISEASE(S): Disease Free

SUBMITTER: Sang-Ho Kwon  

LAB HEAD: Sang-Ho Kwon

PROVIDER: PXD025852 | Pride | 2022-08-12

REPOSITORIES: Pride