Dataset Information

Comparative proteomic analysis of root tubers of Rehmannia glutinosa at different growth stages

ABSTRACT: In this study, roots of Rehmannia glutinosa were used as experimental material, and three tuber roots of Rehmannia glutinosa in extension stage (I), expansion stage (E) and mature stage (M) were selected as samples, The iTRAQ quantitative proteomic technology combined with two-dimensional liquid chromatography and tandem mass spectrometry (2D-LC-MSMS) technology was used to construct the root tuber proteome database of Rehmannia glutinosa, the genes related to the growth and development of Rehmannia glutinosa root tuber were found.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Rehmannia Glutinosa

TISSUE(S): Root

SUBMITTER: XiaoYan Wei

LAB HEAD: Hongying Duan

PROVIDER: PXD025914 | Pride | 2022-02-17

REPOSITORIES: Pride

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Publications

Study of Terpenoid Synthesis and Prenyltransferase in Roots of Rehmannia glutinosa Based on iTRAQ Quantitative Proteomics.

Chen Peilei P Wei Xiaoyan X Qi Qianting Q Jia Wenjing W Zhao Mingwei M Wang Huina H Zhou Yanqing Y Duan Hongying H

Frontiers in plant science 20210804

Rehmannia glutinosa has important medicinal value; terpenoid is one of the main active components in R. glutinosa. In this study, iTRAQ technique was used to analyze the relative abundance of proteins in roots of R. glutinosa, and 6,752 reliable proteins were quantified. GO enrichment results indicated that most proteins were involved in metabolic process or cellular process, 57.63% proteins had catalytic activity, and 65.80% proteins were enriched in membrane-bounded organe ...[more]

PMID: 34421945

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Project description:Cotton (Gossypium hirsutum L.) is one of the most important cash crops worldwide. In semi-arid/arid regions, drought stress causes growth limitation and decrease of yield. Of all the organs of a plant, fine root is the central part consisting of the root system to contribute to plant water and nutrient taken up. However, the research on the molecular mechanism underlying fine root response to soil drought has not been well understood in cotton. To better characterize the proteomic changes of cotton fine roots under drought stress, 70±5% and 40±5% soil relative water content were designed as control (CK) and drought stress (DS) groups, respectively. Tandem mass tags (TMT) technology was used to determine the proteome profiles in fine roots. The proteomic differences between CK and DS were pairwise compared at 0, 30, and 45 days after drought stress (DAD). A total of 11,628 proteins were identified, of which 10,344 proteins contained quantitative information. According to the morphological, physiological, and biochemical characteristics, 30 and 45 DAD were selected as critical stages for further analysis. Results showed that 118 differentially expressed proteins (DEPs) were up-regulated and 105 down-regulated in DS 30 versus CK 30; 662 DEPs were up-regulated, and 611 were down-regulated in DS 45 versus CK 45. The DEP functions were determined for their classified pathways, mainly associated with carbohydrate metabolism, energy metabolism, fatty acid metabolism, amino acid metabolism, and secondary metabolite biosynthesis. DEPs related to phytohormone and stress/defense response were also identified. To verify the accuracy of the TMT results, 20 DEPs were randomly selected for parallel reaction monitoring (PRM) verification. And results showed that the quantitative results of TMT are consistent with those of PRM, which proved that the TMT results of this study are reliable. In this article we describe changes in the protein profiles occurring in response to drought stress in cotton fine roots. Proteomic analyses of plant responses to stressors could lead to the introduction of cotton cultivars with high resistance to drought stress. Such plants would be valuable for high yielding under drought as well as other unfavorable environmental conditions.

Dataset Information

Comparative proteomic analysis of root tubers of Rehmannia glutinosa at different growth stages

Publications

Study of Terpenoid Synthesis and Prenyltransferase in Roots of <i>Rehmannia glutinosa</i> Based on iTRAQ Quantitative Proteomics.

Similar Datasets

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