Dataset Information

Investigation of changes in the proteome and the phosphoproteome of the heart induced by exercise, either in control or in PKP2-deficient hearts in relation to ARVC

ABSTRACT: We performed an unbiased investigation of changes in the proteome and the phosphoproteome of the heart induced by exercise, either in control or in PKP2-deficient hearts. Two pairs of experimental groups were studied: The first pair consisted of control animals and the second pair of animals deficient in PKP2 (PKP2cKO). For both cases, results obtained from sedentary animals were compared to those obtained from animals subjected to six weeks of a running exercise program. We therefore refer to the first pair as “control” and the second pair as “PKP2cKO” and in both cases, the variable was “exercise”. Left ventricular tissue from these animals were subjected to quantitative proteome and phosphoproteome measurements applying tandem mass tag (TMT) labeling and high-resolution mass spectrometry measurements performed on an Orbitrap QExactive mass spectrometer. For phosphoproteomic analyses, peptides were enriched by titanium dioxide chromatography and all peptide samples were pre-fractionated into 12 fractions prior to measurement.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

DISEASE(S): Arrhythmogenic Right Ventricular Cardiomyopathy

SUBMITTER: Navratan Bagwan

LAB HEAD: Alicia Lundby

PROVIDER: PXD025956 | Pride | 2022-11-04

REPOSITORIES: Pride

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Publications

Exercise Causes Arrhythmogenic Remodeling of Intracellular Calcium Dynamics in Plakophilin-2-Deficient Hearts.

van Opbergen Chantal J M CJM Bagwan Navratan N Maurya Svetlana R SR Kim Joon-Chul JC Smith Abigail N AN Blackwell Daniel J DJ Johnston Jeffrey N JN Knollmann Björn C BC Cerrone Marina M Lundby Alicia A Delmar Mario M

Circulation 20220501 19

<h4>Background</h4>Exercise training, and catecholaminergic stimulation, increase the incidence of arrhythmic events in patients affected with arrhythmogenic right ventricular cardiomyopathy correlated with plakophilin-2 (PKP2) mutations. Separate data show that reduced abundance of PKP2 leads to dysregulation of intracellular Ca2+ (Ca2+i) homeostasis. Here, we study the relation between excercise, catecholaminergic stimulation, Ca2+i homeos ...[more]

PMID: 35491884

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Project description:While the salutary effects of exercise training on conduit artery endothelial cells have been reported in animals and humans with cardiovascular risk factors or disease, whether a healthy endothelium is alterable with exercise training is less certain. The purpose of this study was to evaluate the impact of exercise training on transcriptional profiles in normal endothelial cells using a genome-wide microarray analysis. Brachial and internal mammary endothelial gene expression was compared between a group of healthy pigs that exercise-trained for 16-20 weeks (n=8) and a group that remained sedentary (n=8). We found that a total of 130 genes were up regulated and 84 genes down regulated in brachial artery endothelial cells with exercise training. In contrast, a total of 113 genes were up regulated and 31 genes down regulated in internal mammary artery endothelial cells (>1.5-fold and false discovery rate<15%). Although there was an overlap of 66 genes (59 up regulated and 7 down regulated with exercise training) between the brachial and internal mammary arteries, the identified endothelial gene networks and biological processes influenced by exercise training were distinctly different between the brachial and internal mammary arteries. These data indicate that a healthy endothelium is indeed responsive to exercise training and support the concept that the influence of physical activity on endothelial gene expression is not homogenously distributed throughout the vasculature. Brachial and internal mammary endothelial gene expression was compared between a group of healthy pigs that exercise-trained for 16-20 weeks (n=8) and a group that remained sedentary (n=8). The arteries were taken from the same animals, and after quality assessment, so there were 29 total arrays (15 unique pigs), 14 with an array for both the brachial artery and the internal mammary artery (IMA), and the remaining 1 having only brachial. One pig had bad RNA quality and is missing from both IMA and brachical. Therefore, there were 15 IMA (7 SED, 8 EX) and 14 brachial arrays (7 SED, 7 EX) that were used in this study.

Dataset Information

Investigation of changes in the proteome and the phosphoproteome of the heart induced by exercise, either in control or in PKP2-deficient hearts in relation to ARVC

Publications

Exercise Causes Arrhythmogenic Remodeling of Intracellular Calcium Dynamics in Plakophilin-2-Deficient Hearts.

Similar Datasets

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