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Coordinated control of the Aurora B abscission checkpoint by PKCε complex assembly, midbody recruitment and retention

ABSTRACT: The N371 site on PKCe was mutated to TAG in an expression plasmid. This was co-transfected in a 1:1 ratio with a plasmid that encodes both an aaRS optimised to recognise AbK, and the tRNAPyl which recognises the UAG codon.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Steven Lynham

LAB HEAD: Prof. Peter Parker

PROVIDER: PXD025959 | Pride | 2021-11-03

REPOSITORIES: Pride

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Co-ordinated control of the Aurora B abscission checkpoint by PKCε complex assembly, midbody recruitment and retention.

Watson Lisa L Soliman Tanya N TN Davis Khalil K Kelly Joanna J Lockwood Nicola N Yang Xiaoping X Lynham Steven S Scott John D JD Crossland Victoria V McDonald Neil Q NQ Mann David J DJ Armstrong Alan A Eggert Ulrike U Parker Peter J PJ Parker Peter J PJ

The Biochemical journal 20210601 12

A requirement for PKCε in exiting from the Aurora B dependent abscission checkpoint is associated with events at the midbody, however, the recruitment, retention and action of PKCε in this compartment are poorly understood. Here, the prerequisite for 14-3-3 complex assembly in this pathway is directly linked to the phosphorylation of Aurora B S227 at the midbody. However, while essential for PKCε control of Aurora B, 14-3-3 association is shown to be unnecessary for the activity-dependent enrich ...[more]

PMID: 34143863

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