Coordinated control of the Aurora B abscission checkpoint by PKCε complex assembly, midbody recruitment and retention

ABSTRACT: The N371 site on PKCe was mutated to TAG in an expression plasmid. This was co-transfected in a 1:1 ratio with a plasmid that encodes both an aaRS optimised to recognise AbK, and the tRNAPyl which recognises the UAG codon.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Steven Lynham  

LAB HEAD: Prof. Peter Parker

PROVIDER: PXD025959 | Pride | 2021-11-03

REPOSITORIES: Pride