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Multiplexed isobaric quantitative Protein Interaction Reporter (iqPIR) cross-linking on standard proteins

ABSTRACT: Initial demonstration of multiplexed isobaric quantitative Protein Interaction Reporter technology using two multiplexed samples consisting of 5 cross-linked standard proteins including alcohol dehydrogenase 1 (ADH1_YEAST), albumin (ALBU_BOVIN), cytochrome c (CYC_BOVIN), hemoglobin (HBA_HUMAN), and myoglobin (MYG_HORSE).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Equus Caballus (horse) Homo Sapiens (human) Bos Taurus (bovine) Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Juan Chavez

LAB HEAD: James E. Bruce

PROVIDER: PXD026037 | Pride | 2023-03-11

REPOSITORIES: Pride

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Multiplexed Cross-Linking with Isobaric Quantitative Protein Interaction Reporter Technology.

Chavez Juan D JD Keller Andrew A Wippel Helisa H HH Mohr Jared P JP Bruce James E JE

Analytical chemistry 20211209 50

Chemical cross-linking with mass spectrometry (XL-MS) has emerged as a useful technique for interrogating protein structures and interactions. When combined with quantitative proteomics strategies, protein conformational and interaction dynamics can be probed. Quantitative XL-MS has been demonstrated with the use of stable isotopes incorporated metabolically or into the cross-linker molecules. Isotope-labeled cross-linkers have primarily utilized deuterium and rely on MS1-based quantitation of p ...[more]

PMID: 34882395

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