Dataset Information

Investigation of xylan degradation in Thermococcus sp. 2319x1E

ABSTRACT: Thermococcus sp. 2319x1E is able to grow on different mono- or polysaccharides as carbon source, including xylan. To investigate xylan degradation in Thermococcus sp. 2319x1E, cells were grown on 4 different carbon sources for 12 h (mid-exponential phase) and harvested by centrifugation. Using a label-free quantification approach the whole proteomes were analyzed. In another experiment a glycosidase specific activity-based probe (JJB384) was used to isolate specific Thermococcus glycosidases activated during growth on xylan or xylose.

INSTRUMENT(S): Orbitrap Fusion Lumos, LTQ Orbitrap Elite

ORGANISM(S): Unclassified Thermococcus

SUBMITTER: Farnusch Kaschani

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD026056 | Pride | 2022-02-17

REPOSITORIES: Pride

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Publications

Activity-Based Protein Profiling for the Identification of Novel Carbohydrate-Active Enzymes Involved in Xylan Degradation in the Hyperthermophilic Euryarchaeon Thermococcus sp. Strain 2319x1E.

Klaus Thomas T Ninck Sabrina S Albersmeier Andreas A Busche Tobias T Wibberg Daniel D Jiang Jianbing J Elcheninov Alexander G AG Zayulina Kseniya S KS Kaschani Farnusch F Bräsen Christopher C Overkleeft Herman S HS Kalinowski Jörn J Kublanov Ilya V IV Kaiser Markus M Siebers Bettina B

Frontiers in microbiology 20220112

Activity-based protein profiling (ABPP) has so far scarcely been applied in Archaea in general and, especially, in extremophilic organisms. We herein isolated a novel Thermococcus strain designated sp. strain 2319x1E derived from the same enrichment culture as the recently reported Thermococcus sp. strain 2319x1. Both strains are able to grow with xylan as the sole carbon and energy source, and for Thermococcus sp. strain 2319x1E (optimal growth at 85°C, pH 6-7), the inducti ...[more]

PMID: 35095781

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Dataset Information

Investigation of xylan degradation in Thermococcus sp. 2319x1E

Publications

Activity-Based Protein Profiling for the Identification of Novel Carbohydrate-Active Enzymes Involved in Xylan Degradation in the Hyperthermophilic Euryarchaeon <i>Thermococcus</i> sp. Strain 2319x1E.

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