Identification of trans-acting factors mediating mRNA localization

ABSTRACT: Here we aimed to identify protein interacting partners of the (AU)8 motif mediating mRNA localization. For that, RNA carrying this motifs and its mutated version (ATATATATATATATAT ➝ GTACATACATGTACAT) is included in in vitro produced RNA containing 5 boxB sites, the RNA is incubated with mouse brain lysate and fished out using GRNA chromatography. Bound proteins were analysed by mass spectrometry and ELAVL2, ELAVL3 and ELAVl4 identified among the most enriched interactions of AU)8 motif.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Marieluise Kirchner  

LAB HEAD: Philipp Mertins

PROVIDER: PXD026089 | Pride | 2022-11-15

REPOSITORIES: Pride