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Effect of HPV8-E7 expression on the secretome of keratinocytes

ABSTRACT: Human papillomavirus type 8 (HPV8) is associated with the development of non-melanoma skin cancer. In the past we already delved into the mechanisms involved in keratinocyte invasion, showing that the viral E7 oncoprotein is a key player that drives invasion of basal keratinocytes controlled by the extracellular protein fibronectin. To unravel further downstream effects in E7 expressing keratinocytes we now characterized alterations of the secretome of E7 expressing N/TERT keratinocytes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte, Skin

DISEASE(S): Skin Cancer

SUBMITTER: Prerana Wagle

LAB HEAD: Baki Akgül

PROVIDER: PXD026100 | Pride | 2022-02-17

REPOSITORIES: Pride

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Novel Insights Into Cellular Changes in HPV8-E7 Positive Keratinocytes: A Transcriptomic and Proteomic Analysis.

Kirschberg Matthias M Syed Adnan Shahzad AS Dönmez Hanife Güler HG Heuser Sandra S Wilbrand-Hennes Astrid A Alonso Angel A Hufbauer Martin M Akgül Baki B

Frontiers in microbiology 20210906

Human papillomavirus type 8 (HPV8) is associated with the development of non-melanoma skin cancer. In the past we already delved into the mechanisms involved in keratinocyte invasion, showing that the viral E7 oncoprotein is a key player that drives invasion of basal keratinocytes controlled by the extracellular protein fibronectin. To unravel further downstream effects in E7 expressing keratinocytes we now aimed at characterizing gene and protein/phosphoprotein alterations to narrow down on key ...[more]

PMID: 34552568

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Effect of HPV8-E7 expression on the phospho-proteome in keratinocytes

Project description:Human papillomavirus type 8 (HPV8) is associated with the development of non-melanoma skin cancer. In the past we already delved into the mechanisms involved in keratinocyte invasion, showing that the viral E7 oncoprotein is a key player that drives invasion of basal keratinocytes controlled by the extracellular protein fibronectin. To unravel further downstream effects in E7 expressing keratinocytes we now characterized alterations of the phospho-proteome in E7 expressing N/TERT keratinocytes.

2022-02-17 | PXD026099 | Pride

Transcription profiling of human foreskin keratinocytes expressing E6 or E7 compared to normal keratinocytes reveals HPV16 E7 induces EMT

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Smart-Seq of embryos injected with the mRNA of a recombinant OGA

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2024-03-30 | E-MTAB-12981 | biostudies-arrayexpress

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2019-03-18 | PXD012186 | Pride

Expression signatures and cytogenetic aberrations in HPV16 E6, E7 and E6/E7-positive immortalized human epithelial cells

Project description:Identification of genetic/cytogenetic alterations and differentially expressed cellular genes in HPV16 E6, E7 and E6/E7 positive human foreskin keratinocytes Keywords: ordered We used microarrays to identify differentially expressed genes in human foreskin keratinocytes (HFK) transfected with retroviral vectors harboring the human papillomavirus type 16 oncogenes E6, E7, or E6/E7 in comparison to HFK containing the empty vector control pLXSN.

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