Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PfAP2-O Influences Virulence Gene Transcription and Sexual Development in Plasmodium falciparum

ABSTRACT: The project examined the function of the putative transcription factor PfAP2-O from Plasmodium falciparum. This factor besides an essential function in sexual stages also influences variant, virulence associated genes. In a pull down analysis, HA-tagged PfAP2-O was immunoprecipitated and proteins assoaciated were analysed and three differentially appearing protein bands were analysed.

INSTRUMENT(S):

ORGANISM(S): Plasmodium Falciparum (isolate 3d7)

TISSUE(S): Blood

DISEASE(S): Plasmodium Falciparum Malaria

SUBMITTER: Gerhard Wunderlich

LAB HEAD: Gerhard Wunderlich

PROVIDER: PXD026237 | Pride | 2021-06-28

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	15kDa_180221205833.raw	Raw
	30kDa.raw	Raw
	70kDa.raw	Raw
	SupplementaryTable2-ProteomicAnalysis.xlsx	Xlsx

Items per page:

1 - 4 of 4

Similar Datasets

Regulators of sexual commitment in Plasmodium falciparum

Project description:The aim of this study was to identify transcriptonal responses during sexual commitment of P. falciparum mutants unable to form gametocytes.

2020-09-29 | GSE158689 | GEO

Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum [array]

Project description:Malaria transmission requires the conversion of some asexual parasites into sexual forms termed gametocytes. The initial stages of sexual development, including sexually-committed schizonts and sexual rings, remain poorly characterized, in part because only a subset of parasites undergo sexual development and they are morphologically identical to their asexual counterparts. Here we present a system based on conditional expression of PfAP2-G, the master regulator of sexual conversion, for controlled sexual induction. Induction resulted in ~90% sexual conversion, which enables the characterization of early sexual stages without further purification. Transcriptomic analysis identified the alterations that follow pfap2-g activation, including identification of genes that are down-regulated. We also show that the inducible system enables the characterization of committed stages at the phenotypic level. Altogether, the inducible lines will facilitate the study of the initial stages of sexual development, which is important for the development of new strategies to stop malaria transmission.

2020-06-10 | GSE135005 | GEO

Lysophosphatidylcholine regulates sexual stage differentiation in the human malaria parasite Plasmodium falciparum

Project description:Purpose: The goal of this study was to identify genes associated with increased sexual differentiation in P. falciparum. Method: RNAseq analysis of two different P. falciparum strains (NF54 and Pf2004/164tdTom) cultured under sexual differentiation-inducing (-SerM) and non-inducing (-SerM/LysoPC and -SerM/Serum) conditions Results: We found a total of 342 genes significantly induced in response to LysoPC depletion, thereby determining changes associated with sexual differentiation and limiting Kenndedy pathway substrates.

2017-11-16 | GSE104114 | GEO

Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum. [ChIP-Seq]

2020-06-10 | GSE135002 | GEO

Changes in heterochromatin distribution during the initial stages of sexual development in Plasmodium falciparum

Project description:In the malaria parasite Plasmodium falciparum, changes in the distribution of heterochromatin at specific loci regulate the expression of clonally variant genes (CVGs), which play fundamental roles in host-parasite interactions. In addition to differences in heterochromatin between individual parasites associated with variant expression of CVGs, changes in heterochromatin distribution associated with different developmental stages have been described. However, while stage II/III gametocytes (sexual precursors) have an altered distribution of heterochromatin compared with asexual blood stages, it is unclear if heterochromatin changes already occur at the very early stages of sexual development. Here we used a transgenic parasite line in which sexual conversion can be conditionally induced to characterise the changes in heterochromatin distribution associated with the earliest stages of sexual development. We found that there were essentially no changes in sexual rings or stage I gametocytes compared with asexual blood stages, and remodelling of heterochromatin started at stage II or III of gametocyte development, with changes similar to those previously described in parasites of a different genetic background. Therefore, global redistribution of heterochromatin does not appear to play a role in the initial steps of sexual development. We also found that, for several genes, changes in expression associated with sexual development precede changes in the heterochromatin at their locus.

2024-10-09 | GSE252334 | GEO

Regulators of sexual commitment in Plasmodium falciparum

Project description:Regulators of sexual commitment in Plasmodium falciparum

| PRJNA666191 | ENA

Probing Plasmodium falciparum sexual differentiation at the single cell level

Project description:Malaria parasites go through major transitions during their complex life cycle, yet the underlying differentiation pathways remain obscure. Here we apply single cell transcriptomics to unravel events that initiate sexual development in preparation for transmission of the parasite from human to mosquito. This proof-of-concept study provides a template to capture transcriptional diversity in heterogeneous parasite populations, with major implications for our understanding of parasite biology and the ongoing malaria elimination campaign.

2017-03-11 | GSE96066 | GEO

Plasmodium falciparum PHIL1 LC-MS/MS

Project description:Plasmodium falciparum sexual-stage parasites expressing an epitope-tagged PHIL1 protein were analysed by co-IP and subsequent LC-MS/MS as

2017-09-28 | PXD007564 | Pride

Sexual Regeneration RNAseq

Project description:Sexual Regeneration RNAseq

| PRJNA421831 | ENA

Sexual Regeneration RNAseq

Project description:Sexual Schmidtea mediterranea Trunk Regeneration. Sexual biotype Schmidtea mediterranea trunk fragments were sequenced at 0 day, 3 day, 5 day and 7 day post amputation along with whole worm juvenile animals in order identify sexual specific transcripts. Juvenile worms lack sex organs, testes and ovaries present in D0 trunk fragments. Days 3-7 represent time points of degeneration and regeneration of the sexual organs, testes and ovaries.

2017-12-11 | GSE107869 | GEO