Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: Richard Imre
LAB HEAD: Dea Slade
PROVIDER: PXD026292 | Pride | 2022-02-17
REPOSITORIES: Pride
Appel Lisa-Marie LM Franke Vedran V Bruno Melania M Grishkovskaya Irina I Kasiliauskaite Aiste A Kaufmann Tanja T Schoeberl Ursula E UE Puchinger Martin G MG Kostrhon Sebastian S Ebenwaldner Carmen C Sebesta Marek M Beltzung Etienne E Mechtler Karl K Lin Gen G Vlasova Anna A Leeb Martin M Pavri Rushad R Stark Alexander A Akalin Altuna A Stefl Richard R Bernecky Carrie C Djinovic-Carugo Kristina K Slade Dea D
Nature communications 20211019 1
The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) is a regulatory hub for transcription and RNA processing. Here, we identify PHD-finger protein 3 (PHF3) as a regulator of transcription and mRNA stability that docks onto Pol II CTD through its SPOC domain. We characterize SPOC as a CTD reader domain that preferentially binds two phosphorylated Serine-2 marks in adjacent CTD repeats. PHF3 drives liquid-liquid phase separation of phosphorylated Pol II, colocalizes wi ...[more]