Proteomics

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Evolution of enhanced innate immune evasion by the SARS-CoV-2 B.1.1.7 UK variant

ABSTRACT: Here we use unbiased abundance proteomics and phosphoproteomics to assess global changes to host and viral proteins in Calu-3 cells at 10 and 24 hours post infection with either the B.1.1.7 UK variant or early-lineage SARS-CoV-2 viruses VIC and IC19.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human) Severe Acute Respiratory Syndrome Coronavirus 2

TISSUE(S): Lung, Epithelial Cell

SUBMITTER: Mehdi Bouhaddou  

LAB HEAD: Nevan J. Krogan

PROVIDER: PXD026302 | Pride | 2022-01-20

REPOSITORIES: Pride

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Publications

Evolution of enhanced innate immune evasion by SARS-CoV-2.

Thorne Lucy G LG   Bouhaddou Mehdi M   Reuschl Ann-Kathrin AK   Zuliani-Alvarez Lorena L   Polacco Ben B   Pelin Adrian A   Batra Jyoti J   Whelan Matthew V X MVX   Hosmillo Myra M   Fossati Andrea A   Ragazzini Roberta R   Jungreis Irwin I   Ummadi Manisha M   Rojc Ajda A   Turner Jane J   Bischof Marie L ML   Obernier Kirsten K   Braberg Hannes H   Soucheray Margaret M   Richards Alicia A   Chen Kuei-Ho KH   Harjai Bhavya B   Memon Danish D   Hiatt Joseph J   Rosales Romel R   McGovern Briana L BL   Jahun Aminu A   Fabius Jacqueline M JM   White Kris K   Goodfellow Ian G IG   Takeuchi Yasu Y   Bonfanti Paola P   Shokat Kevan K   Jura Natalia N   Verba Klim K   Noursadeghi Mahdad M   Beltrao Pedro P   Kellis Manolis M   Swaney Danielle L DL   García-Sastre Adolfo A   Jolly Clare C   Towers Greg J GJ   Krogan Nevan J NJ  

Nature 20211223 7897

The emergence of SARS-CoV-2 variants of concern suggests viral adaptation to enhance human-to-human transmission<sup>1,2</sup>. Although much effort has focused on the characterization of changes in the spike protein in variants of concern, mutations outside of spike are likely to contribute to adaptation. Here, using unbiased abundance proteomics, phosphoproteomics, RNA sequencing and viral replication assays, we show that isolates of the Alpha (B.1.1.7) variant<sup>3</sup> suppress innate immu  ...[more]

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