G-CSF mediated signalling in 32D cells

ABSTRACT: G-CSF signalling has been extensively characterized in myeloid progenitor cells but the activation dynamics downstream of the receptor at different stages of cell differentiation in wild-type G-CSFR or in proliferating cells that harbor the d715 mutation has not been been investigated at the phosphoproteome/proteome level. Proteome and phosphoproteome profiling using 10 plexTMT has been performed on 32D cells stimulated with G-CSF on d715 mutant cells, wild type cells on day 0, day 3 and day 6 of cell differentiation. Each sample on each day has 5 time points sampled at t=0,5,10,15,30 minutes post G-CSF stimulation.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: James Wright  

LAB HEAD: Jyoti Choudhary

PROVIDER: PXD026317 | Pride | 2026-06-22

REPOSITORIES: Pride