Dataset Information

Schistosoma mansoni cercarial head and tail LC-MSMS

ABSTRACT: Label-Free Quantitative analysis of the proteome of infectious cercaria of the species Schistosoma mansoni. The proteome of the cercarial head and cercarial tail were compared to elucidate differences in stored proteins that control biological function during development.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Schistosoma Mansoni (blood Fluke)

TISSUE(S): Head, Tail

SUBMITTER: Emmitt Jolly

LAB HEAD: Emmitt Randolph Jolly

PROVIDER: PXD026435 | Pride | 2022-02-17

REPOSITORIES: Pride

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Publications

Multiomic analysis of Schistosoma mansoni reveals unique expression profiles in cercarial heads and tails.

Hagerty James R JR Kim Hyung Chul HC Jolly Emmitt R ER

Communications biology 20210712 1

Schistosomes require both molluscan and mammalian hosts for development. The larval cercaria exits the snail host and swims to identify and invade the mammalian host. The cercaria has two macrostructures, the head and the tail. The head invades the host, where it matures into an adult worm. The tail is lost after host invasion. Translation in the cercaria differs in each macrostructure, with higher levels of translation in the cercarial tail and little to no translational activity in the cercari ...[more]

PMID: 34253841

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Project description:An increased serum alkaline phosphatase concentration is known to be associated with a negative prognosis in canine and human osteosarcoma. To expand upon previous studies regarding the biological relevance of increased serum alkaline phosphatase as a negative prognostic factor, xenogeneic heterotopic transplants were performed using six canine primary osteosarcoma cell lines generated from patients with differing serum alkaline phosphatase concentrations (3 normal and 3 increased). Three of the six cell lines were capable of generating tumors and tumor formation was independent of the serum alkaline phosphatase status of the cell line. Microarray analysis identified 379 genes as being differentially-expressed between the tumorigenic and non-tumorigenic cell lines. Frizzled-6 was up-regulated to the greatest extent (7.78 fold) in tumorigenic cell lines compared to non-tumorigenic cell lines. Frizzled-6, a co-receptor for Wnt ligands has been associated with enhanced tumor-initiating cells and poor prognosis for other tumors. The increased expression of frizzled-6 was confirmed by QPCR and Western blot analysis. Additionally, the tumorigenic cell lines also had an increase in the percentage of side population cells compared to non-tumorigenic cell lines (5.89% versus 1.58%, respectively). There were no differences in tumorigenicity, frizzled-6, or percentage of side population cells noted between osteosarcoma cell lines generated from patients of differing serum alkaline phosphatase concentration. However, to our knowledge this is the first study to identified frizzled-6 as a possible marker of osteosarcoma cell populations with enhanced tumorigenicity and side population cells. Future work will focus on defining the role of frizzled-6 in osteosarcoma tumorigenesis and tumor-initiating cells. A total of six canine primary osteosarcoma cell lines were used in this study. Three cell lines were capable of forming tumors when transplanted into mice (tumorigenic) and three cell lines were not capable of forming tumors upon transplant into mice (non-tumorigenic). The gene expression data is from the primary cell lines, not the transplanted cells. There were no reference cell lines or controls used in this study.

Dataset Information

Schistosoma mansoni cercarial head and tail LC-MSMS

Publications

Multiomic analysis of Schistosoma mansoni reveals unique expression profiles in cercarial heads and tails.

Similar Datasets

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