HDX-MS Analysis of CD47 Conformational Dynamics

ABSTRACT: CD47 is the only 5-transmembrane (5-TM) spanning receptor of the immune system. Its extracellular domain (ECD) is a cell surface ‘marker of self’ that binds SIRPα and inhibits macrophage phagocytosis, and cancer immuno-therapy approaches in clinical trials are focused on blocking CD47/SIRPα interaction. Using hydrogen-deuterium exchange we show that CD47’s ECLR architecture, comprised of two extracellular loops and the SWF loop, creates a molecular environment stabilizing the ECD for presentation on the cell surface.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Richard Huang  

LAB HEAD: Richard Huang

PROVIDER: PXD026458 | Pride | 2021-07-15

REPOSITORIES: Pride