Dataset Information

Proteomic and mutant analysis of hydrogenase gene hypE in symbiotic nitrogen fixation in Mesorhizobium huakuii

ABSTRACT: Hydrogenases catalyze the simple yet importantredox reaction between protons and electrons and H2, thus, mediate interactions between microorganisms and their chemical environment. A mutation in M. huakuii hypE gene was generated by homologous recombination. The hypE mutant grew a little faster than its parental 7653R. Real-time quantitative PCR showed that the hypE gene expression is significantly upregulated in all the detected stages of nodule development. The wild type contained normal spherical shape of the nodules, while the hypE mutant elicited small-size nodules. Plants inoculated with mutant HKhypE showed a significantly more than 47% decrease in acetylene reduction activity compared to the wild-type 7653R. Proteomics analysis showed seven proteins were up-regulated and eighty-two proteins were down-regulated in hypE triple mutant bacteroids.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mesorhizobium Huakuii

TISSUE(S): Root Nodule

SUBMITTER: Guojun Cheng

LAB HEAD: Guojun Cheng

PROVIDER: PXD026564 | Pride | 2023-10-24

REPOSITORIES: Pride

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Publications

Proteomic and Mutant Analysis of Hydrogenase Maturation Protein Gene hypE in Symbiotic Nitrogen Fixation of Mesorhizobium huakuii.

Long Songhua S Su Min M Chen Xiaohong X Hu Aiqi A Yu Fuyan F Zou Qian Q Cheng Guojun G

International journal of molecular sciences 20230808 16

Hydrogenases catalyze the simple yet important redox reaction between protons and electrons and H2, thus mediating symbiotic interactions. The contribution of hydrogenase to this symbiosis and anti-oxidative damage was investigated using the M. huakuii hypE (encoding hydrogenase maturation protein) mutant. The hypE mutant grew a little faster than its parental 7653R and displayed decreased antioxidative capacity under H2O2-induc ...[more]

PMID: 37628715

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Dataset Information

Proteomic and mutant analysis of hydrogenase gene hypE in symbiotic nitrogen fixation in Mesorhizobium huakuii

Publications

Proteomic and Mutant Analysis of Hydrogenase Maturation Protein Gene <i>hypE</i> in Symbiotic Nitrogen Fixation of <i>Mesorhizobium huakuii</i>.

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