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Parallel reaction monitoring (PRM) analysis for protein expression

ABSTRACT: According to a previous study on the microscopic examination of stem barks in ramie, bast fibers from different parts of stem bark showed distinct differences at the developmental level: specifically, the barks in the top part of stem (TPS) did not initiate fiber growth, whereas barks in the middle part of stem (MPS) had a large number of fibers with thickening secondary walls. We have performed the phosphoproteome analysis for the tissues of TPS and MPS, and then carried out the comparison of phosphorylation level of proteins between two tissues, thereby to identified differentially phosphorylated proteins. These datasets represent the raw UHPLC spectra for phosphoproteome of ramie TPS and MPS.

INSTRUMENT(S): LTQ

ORGANISM(S): Boehmeria Nivea (chinese Grass) (urtica Nivea)

TISSUE(S): Bark

SUBMITTER: Zeng Zheng

LAB HEAD: Zeng Zheng

PROVIDER: PXD026598 | Pride | 2022-02-17

REPOSITORIES: Pride

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Identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis.

Li Fu F Zeng Zheng Z Huang Renyan R Wang Yanzhou Y Liu Touming T

BMC genomics 20211202 1

<h4>Background</h4>Ramie is an important fiber-producing crop in China, and its fibers are widely used as textile materials. Fibers contain specialized secondary cellular walls that are mainly composed of cellulose, hemicelluloses, and lignin. Understanding the mechanism underlying the secondary wall biosynthesis of fibers will benefit the improvement of fiber yield and quality in ramie.<h4>Results</h4>Here, we performed a proteomic analysis of the bark from the top and middle parts of the stem, ...[more]

PMID: 34856929

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Proteome and differential proteomic analysis of ramie stem bark

Project description:According to a previous study on the microscopic examination of stem barks in ramie, bast fibers from different parts of stem bark showed distinct differences at the developmental level: specifically, the barks in the top part of stem (TPS) did not initiate fiber growth, whereas barks in the middle part of stem (MPS) had a large number of fibers with thickening secondary walls. We have performed the Proteome analysis for the tissues of TPS and MPS, and then carried out the comparison of Proteomic level of proteins between two tissues, thereby to identified differentially Proteomic proteins. These datasets represent the raw UHPLC spectra for Proteome of ramie TPS and MPS.

2022-02-17 | PXD026596 | Pride

Phosphoproteome of ramie stem barks

Project description:According to a previous study on the microscopic examination of stem barks in ramie, bast fibers from different parts of stem bark showed distinct differences at the developmental level: specifically, the barks in the top part of stem (TPS) did not initiate fiber growth, whereas barks in the middle part of stem (MPS) had a large number of fibers with thickening secondary walls. We have performed the phosphoproteome analysis for the tissues of TPS and MPS, and then carried out the comparison of phosphorylation level of proteins between two tissues, thereby to identified differentially phosphorylated proteins. These datasets represent the raw UHPLC spectra for phosphoproteome of ramie TPS and MPS.

2021-03-21 | PXD024896 | iProX

Ubiquitylome of ramie stem bark

Project description:According to a previous study on the microscopic examination of stem barks in ramie, bast fibers from different parts of stem bark showed distinct differences at the developmental level: specifically, the barks in the top part of stem (TPS) did not initiate fiber growth, whereas barks in the middle part of stem (MPS) had a large number of fibers with thickening secondary walls. We have performed the Ubiquitylome analysis for the tissues of TPS and MPS, and then carried out the comparison of Ubiquitination level of proteins between two tissues, thereby to identified differentially Ubiquitinated proteins. These datasets represent the raw UHPLC spectra for Ubiquitylome of ramie TP S and MPS.

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2022-02-28 | PXD002026 | Pride

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2020-02-26 | PXD017363 | Pride

Comparison of Akt activated cells and placental mesenchymal progenitors

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2013-08-30 | E-MEXP-3725 | biostudies-arrayexpress

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Project description:It is challenging to derive totipotent stem cells in vitro that functionally and molecularly resemble cells from totipotent embryos. Here, we report that a chemical cocktail enables the derivation of totipotent-like stem cells, designated as totipotent potential stem (TPS) cells, from 2-cell mouse embryos and extended pluripotent stem cells that can be stably maintained long-term in vitro. TPS cells shared features with 2-cell mouse embryos in terms of totipotency markers, transcriptome, chromatin accessibility and DNA methylation patterns. In vivo chimeric assays show that these cells have embryonic and extraembryonic developmental potentials at the single cell level. Moreover, TPS cells can be induced into blastocyst-like structures resembling preimplantation mouse blastocysts. Mechanistically, inhibition of HDAC1/2 and DOT1L activity and activation of RARγ signaling are important for inducing and maintaining totipotent features of TPS cells. Our study opens up a path toward fully capturing totipotent stem cells in vitro.

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