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Fecal Transplant from Roux-Y-Gastric-Bypass rat model into a diet induced obesity rat model

ABSTRACT: Investigating alterations the intestinal microbiome in a diet induced obesity (DIO) rat model after fecal transplant from rats, which underwent Roux-Y-Gastric-Bypass surgery (RYGB). The microbiomes of the RYGB-donor rats, the DIO rats, and DIO rats after receiving the fecal transplant from the RYGB rats. As controls lean rats as well as lean, RYGB and DIO rats after antibiotics treatment were used.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

SUBMITTER: Sven Haange

LAB HEAD: Nico Jehmlich

PROVIDER: PXD026606 | Pride | 2022-08-12

REPOSITORIES: Pride

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Functional changes of the gastric bypass microbiota reactivate thermogenic adipose tissue and systemic glucose control via intestinal FXR-TGR5 crosstalk in diet-induced obesity.

Münzker Julia J Haase Nadine N Till Andreas A Sucher Robert R Haange Sven-Bastiaan SB Nemetschke Linda L Gnad Thorsten T Jäger Elisabeth E Chen Jiesi J Riede Sjaak J SJ Chakaroun Rima R Massier Lucas L Kovacs Peter P Ost Mario M Rolle-Kampczyk Ulrike U Jehmlich Nico N Weiner Juliane J Heiker John T JT Klöting Nora N Seeger Gudrun G Morawski Markus M Keitel Verena V Pfeifer Alexander A von Bergen Martin M Heeren Joerg J Krügel Ute U Fenske Wiebke K WK

Microbiome 20220624 1

<h4>Background</h4>Bariatric surgery remains the most effective therapy for adiposity reduction and remission of type 2 diabetes. Although different bariatric procedures associate with pronounced shifts in the gut microbiota, their functional role in the regulation of energetic and metabolic benefits achieved with the surgery are not clear.<h4>Methods</h4>To evaluate the causal as well as the inherent therapeutic character of the surgery-altered gut microbiome in improved energy and metabolic co ...[more]

PMID: 35739571

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Transcription profiling of rats subjected to Roux-en-Y gastruc bypass to characterize of weight loss and weight regain mechanisms

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Project description:Albuminuria is significantly reduced following Roux-en-Y gastric bypass (RYGB) surgery, suggesting a renoprotective effect of the intervention. Herein, we assess the relative impact of RYGB and RYGB equivalent non-surgical weight loss and glycaemic improvement on glomerular injury and global renal transcriptomic responses in the Zucker diabetic fatty rat (ZDF) model of diabetic nephropathy. We coined the term "medical bypass" (MB) to describe the intensive diet and pharmacotherapy based non-surgical intervention Adult ZDF rats underwent sham surgery (n=15) or RYGB (n=9). Nine sham-operated rats were calorie restricted and received insulin, liraglutide, metformin, ramipril, rosuvastatin and fenofibrate for 2 months (MB). Zucker fa/+ rats acted as healthy controls throughout. Bodyweight, glycaemia, albuminuria and glomerular injury specifically podocyte number, density and ultrastructure were assessed at follow up. Renal transcriptomes were compared by RNA-seq. RYGB resulted in 20-30% weight loss, normalized glycaemia and albuminuria and reduced indices of glomerular injury, specifically podocyte injury (foot process effacement). RYGB equivalent outcomes were obtained on all parameters following MB.

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