Dataset Information

The cell wall proteome of Craterostigma plantagineum cell cultures habituated to dichlobenil and isoxaben

ABSTRACT: The remarkable desiccation tolerance of the vegetative tissues in the resurrection species Craterostigma plantagineum (Hochst.) is favored by its unique cell wall folding mechanism that allows the ordered and reversible shrinking of the cells without damaging neither the cell wall nor the underlying plasma membrane. The ability to withstand extreme drought is also maintained in abscisic acid pre-treated calli, which can be cultured both on solid and in liquid culture media. Cell wall research has greatly advanced thanks to the use of inhibitors affecting the biosynthesis of e.g. cellulose, since they allowed the identification of the compensatory mechanisms underlying habituation. Considering the innate cell wall plasticity of C. plantagineum, the goal of this investigation was to understand whether habituation to the cellulose biosynthesis inhibitors dichlobenil and isoxaben entailed or not identical mechanisms as known for non-resurrection species and to decipher the cell wall proteome of habituated cells. The results showed that exposure of C. plantagineum calli/cells triggered abnormal phenotypes as reported in non-resurrection species. Additionally, the data demonstrated that it was possible to habituate Craterostigma cells to dichlobenil and isoxaben and that gene expression and proteomics did not follow the same trend. Shotgun and gel-based proteomics revealed a common set of proteins induced upon habituation, but also identified candidates solely induced by habituation to one of the two inhibitors. Finally, it is hypothesized that alterations in auxin levels are responsible for the increased abundance of cell wall-related proteins upon habituation.

INSTRUMENT(S):

ORGANISM(S): Craterostigma Plantagineum

SUBMITTER: Sebastien Planchon

LAB HEAD: Gea Guerriero

PROVIDER: PXD026723 | Pride | 2022-02-17

REPOSITORIES: Pride

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The Cell Wall Proteome of <i>Craterostigma plantagineum</i> Cell Cultures Habituated to Dichlobenil and Isoxaben.

Guerriero Gea G Achen Charles C Xu Xuan X Planchon Sébastien S Leclercq Céline C CC Sergeant Kjell K Berni Roberto R Hausman Jean-Francois JF Renaut Jenny J Legay Sylvain S

Cells 20210902 9

The remarkable desiccation tolerance of the vegetative tissues in the resurrection species <i>Craterostigma plantagineum</i> (Hochst.) is favored by its unique cell wall folding mechanism that allows the ordered and reversible shrinking of the cells without damaging neither the cell wall nor the underlying plasma membrane. The ability to withstand extreme drought is also maintained in abscisic acid pre-treated calli, which can be cultured both on solid and in liquid culture media. Cell wall rese ...[more]

PMID: 34571944

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Project description:Plant molecular improvement group at University of Shizuoka, Japan, previously identified 18 potential salt tolerance mutants using an activation-tagging approach at the cellular level. In one of these mutants, stc8, two genes AtbHLH106 and AtCRK1 were activated adjacent to the borders of T-DNA. AtbHLH106 is known to regulate salt stress by interacting with the G-box (CACGTG) present in the promoters of abiotic stress responsive genes. Here we report that AtCRK1 contributes to salt-stress regulation in Arabidopsis. In this study, TAIL-PCR revealed that AtCRK1 was present 5567 bp from the RB of the T-DNA and activated on both MS without salt and MS supplemented with 150 mM NaCl. Real-time PCR showed that AtCRK1 was enhanced 1.7 times on MS without salt and 2.7 times on MS with 150 mM NaCl. Quantitative expression in different tissues of plant and calli showed that AtCRK1 was highly expressed in leaves and flowers and least expressed in silique. In addition, expression analysis at different levels of NaCl showed that AtCRK1 was salt-inducible. Expression of AtCRK1 was significantly reduced in KO lines and the KO lines showed sensitive phenotype under salt stress, compared with Col-0 plants. We demonstrated that overexpressed calli of AtCRK1 were tolerant to NaCl and ABA, compared with control calli, and the survival percentage of overexpression lines was higher than in KO and wild-type plants. On vertical plates, shoots of overexpression lines did not show significantly stress-tolerant phenotypes, compared with wildtype and KO plants, but roots of overexpression plants were still longer than KO and wildtype plants. Furthermore, microarray analysis of stc8 mutant and KO lines showed that activation of AtCRK1 leads to upregulation of several heat shock proteins: myoinositol phosphate synthase gene, cold binding factor, ABC transporters, and CAT genes. Together, these findings indicate that AtCRK1 plays an important role during abiotic stress in plants through regulation of several abiotic responsive genes.

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