Dataset Information

Investigation of cell wall proteins of Camellia sinensis leaves by combining cell wall proteomics and N-glycoproteomics

ABSTRACT: Cell wall proteins (CWPs) probably play a vital role in fluoride accumulation/detoxification of C. sinensis leaves. However, there has been a lack in CWP identification and characterization up to now. This study is aimed to characterize cell wall proteome of C. sinensis leaves and to develop more CWPs related to stress response. Cell wall proteomics and N-glycoproteomics was employed to investigate CWPs. CWPs were extracted by sequential salt buffers, while N-glycoproteins were enriched by hydrophilic interaction chromatography method, and subsequently all proteins were subjected to UPLC-MS/MS analysis. A total of 501 CWPs and 195 CWPs were identified respectively by cell wall proteomics and N-glycoproteomics profiling with 118 CWPs in common. Among identified CWPs, proteins acting on cell wall polysaccharides, proteases and oxidoreductases represent the top three functional groups. This was the first large-scale investigation of CWPs in C. sinensis.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Camellia Sinensis

TISSUE(S): Leaf

SUBMITTER: Yanli Liu

LAB HEAD: Yanli Liu

PROVIDER: PXD026772 | Pride | 2022-02-17

REPOSITORIES: Pride

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Investigation of cell wall proteins of C. sinensis leaves by combining cell wall proteomics and N-glycoproteomics.

Liu Yanli Y Ma Linlong L Cao Dan D Gong Ziming Z Fan Jing J Hu Hongju H Jin Xiaofang X

BMC plant biology 20210820 1

<h4>Background</h4>C. sinensis is an important economic crop with fluoride over-accumulation in its leaves, which poses a serious threat to human health due to its leaf consumption as tea. Recently, our study has indicated that cell wall proteins (CWPs) probably play a vital role in fluoride accumulation/detoxification in C. sinensis. However, there has been a lack in CWP identification and characterization up to now. This study is aimed to characterize cell wall proteome of C. sinensis leaves a ...[more]

PMID: 34416854

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Project description:We have used the citrus GeneChip array (GPL5731) to survey the transcription profiles of sweet orange in response to the bacterial pathogens Xanthomonas axonopodis pv. citri (Xac) and Xanthomonas axonopodis pv. aurantifolii (Xaa). Xac is the causal agent of the citrus canker disease on a wide range of citrus species, including sweet oranges (Citrus sinensis). On the other hand, Xaa is pathogenic to Mexican lime (Citrus aurantifolia) only, and in sweet orange it triggers a defense response. In order to identify the genes induced during the defense response (Xaa-responsive genes) or citrus canker development (Xac-responsive genes), we conducted microarrays hybridization experiments at 6 and 48 hours after bacterial infiltration (habi). The analysis revealed that genes commonly modulated by Xac and Xaa are associated with basal defenses normally triggered by pathogen-associated molecular patterns, including those involved in reactive oxygen species production and lignification. Significantly, Xac-infected leaves showed considerable changes in the transcriptional profiles of defense-, cell wall-, vesicle trafficking- and cell growth-related genes between 6 and 48 habi. This is consistent with the notion that Xac suppresses host defenses near the beginning of the infection and simultaneously changes the physiological status of the host to promote cell enlargement and division. Finally, Xaa triggered a MAP kinase signaling pathway involving WRKY and ethylene-responsive transcriptional factors known to activate downstream defense genes. Keywords: Comprehensive transcriptional analysis of the Citrus-Xanthomonas interaction Adult leaves of sweet orange were infiltrated with the bacterial suspensions or water (mock control). Two stages were selected after bacterial infiltration for RNA extraction and hybridization on Affymetrix microarrays. In total, these experiments consist of two biological replicates of six samples: water-infiltrated leaves, Xaa-infiltrated leaves and Xac-infiltrated leaves, at both 6 and 48 (habi).

Dataset Information

Investigation of cell wall proteins of Camellia sinensis leaves by combining cell wall proteomics and N-glycoproteomics

Publications

Investigation of cell wall proteins of C. sinensis leaves by combining cell wall proteomics and N-glycoproteomics.

Similar Datasets

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