CD68+ region of rat scar tissue by AutoSTOMP

ABSTRACT: To test the ability of the Automated Spatially Targeted Optical Micro Proteomics (AutoSTOMP) protocol to selectively biotinylate structures of interest within tissue sections we first examined a rat myocardial infarction model. In this model, trauma caused by ligation and infiltrating immune cells causes fibroblast activation and deposition of scar tissue that ultimately impairs cardiac function. Macrophages are thought to play a role in inflammatory regulation and damaged cell turnover in the tissue. We decide to grab the proteome of the macrophage rich regions.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart

SUBMITTER: BOCHENG YIN  

LAB HEAD: Sarah E. Ewald

PROVIDER: PXD026818 | Pride | 2021-08-10

REPOSITORIES: Pride